HAUSP promoted the growth of glioma cells in vitro and in vivo via stabilizing NANOG

doi:10.1016/j.prp.2020.152883

	HAUSP promoted the growth of glioma cells in vitro and in vivo via stabilizing NANOG
	Cheng, Chuan-dong 1,2,3,4; Dong, Yong-fei 3,4; Niu, Wan-xiang 3,4; Niu, Chao-shi 3,4
刊名	PATHOLOGY RESEARCH AND PRACTICE
	2020-04-01
卷号	216
关键词	HAUSP Glioma cell NANOG Cellular behavior Nude mice xenograft
ISSN号	0344-0338
DOI	10.1016/j.prp.2020.152883
通讯作者	Niu, Chao-shi(niuchaoshi@126.com)
英文摘要	Objective: To investigate the role and mechanisms of HAUSP (Herpesvirus Associated Ubiquitin Specific Protease) and NANOG in pathogenesis of malignant human gliomas progression. Methods: Lentivirus-mediated HAUSP over-expression and RNAiHAUSP mediated HAUSP down-regulation were established in the glioma cells (U87 and U251 cell lines). Firstly, Real-time qPCR, western-blot (WB) and immunofluorescence staining were performed to detect mRNA levels, protein expressions and deposition of HAUSP and NANOG in the glioma cells, respectively. Then cell proliferation, invasion, apoptosis and xenograft tumor growth in nude mice were assessed by using cell counting kit-8 (CCK-8) assay, transwell assay, flow cytometry (FCM) and Hematoxylin-Eosin (HE) staining. Results: We first demonstrated HAUSP was significantly increased in lentivirus- mediated HAUSP over-expression cells compared to the Control group. HAUSP over-expression could upregulate genes involved in proliferation and invasion such as NANOG. However, the mRNA of NANOG had no significant changes. Similarly, in RNAiHAUSP mediated HAUSP down-regulation group, HAUSP were significantly decreased compared to the Control group. Simultaneously, NANOG protein were decreased significantly, which decreased the proliferation and invasion, increased the apoptosis rate of glioma cells. Finally, low expression of HAUSP could suppress xenograft tumors growth in nude mice in different periods. Conclusion: This study revealed that HAUSP-NANOG pathway is a key target to inhibit glioma cells proliferation, and NANOG play important role in the formation and evolution of glioma cells. The regulation of HAUSP could change the biological activity of glioma cells through regulate NANOG expression.
资助项目	National Natural Science Foundation of China[81172407] ; Science and Technology Project grant from Anhui Province[1508085QHl84] ; Science and Technology Project grant from Anhui Province[1606c08235] ; Science and Technology Project grant from Anhui Province[1604a0802069] ; Fundamental Research Fund for Central Universities[WK 9110000032]
WOS关键词	STEM-CELLS ; PATHWAY ; P53 ; EXPRESSION ; CANCER ; ENZYME ; PTEN
WOS研究方向	Pathology
语种	英语
出版者	ELSEVIER GMBH
WOS记录号	WOS:000523598700007
资助机构	National Natural Science Foundation of China ; Science and Technology Project grant from Anhui Province ; Fundamental Research Fund for Central Universities
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103705]
专题	中国科学院合肥物质科学研究院
通讯作者	Niu, Chao-shi
作者单位	1.Univ Sci & Technol China, Hefei 230036, Anhui, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Neurosurg, Div Life Sci & Med, Hefei 230036, Anhui, Peoples R China 4.Anhui Prov Key Lab Brain Funct & Brain Dis, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Chuan-dong,Dong, Yong-fei,Niu, Wan-xiang,et al. HAUSP promoted the growth of glioma cells in vitro and in vivo via stabilizing NANOG[J]. PATHOLOGY RESEARCH AND PRACTICE,2020,216.
APA	Cheng, Chuan-dong,Dong, Yong-fei,Niu, Wan-xiang,&Niu, Chao-shi.(2020).HAUSP promoted the growth of glioma cells in vitro and in vivo via stabilizing NANOG.PATHOLOGY RESEARCH AND PRACTICE,216.
MLA	Cheng, Chuan-dong,et al."HAUSP promoted the growth of glioma cells in vitro and in vivo via stabilizing NANOG".PATHOLOGY RESEARCH AND PRACTICE 216(2020).