An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome

doi:10.1016/j.talanta.2020.120941

	An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome
	Yang, Linlin 1,2; Zhao, Jun 1,2; Wang, Jianping 1,2; Han, Guangmei 1,2,5; Liu, Bianhua 1,2; Zhang, Wei 3; Fu, Yao 4; Han, Ming-Yong 1,2; Wang, Zhenyang 1,2; Zhang, Zhongping 1,2,5
刊名	TALANTA
	2020-08-15
卷号	216
关键词	Fluorescent probe Lysosome Autolysosome Azacyclo localization Cell imaging
ISSN号	0039-9140
DOI	10.1016/j.talanta.2020.120941
通讯作者	Han, Guangmei(gmhan@iim.ac.cn) ; Wang, Zhenyang(zywang@iim.ac.cn)
英文摘要	Understanding lysosome-related physiology needs specific lysosome probes to track the biological processes of lysosome in living cells. Here, we report an azacyclo-modified fluorescent probe that has a large Stokes shift, good photostability and negligible cytotoxicity for highly specific labeling of lysosome and autolysosome in living cells. The probes with different kinds of azacyclo groups on parent dye dansyl are screened to show that dansyl-cycleanine (DNS-C) with four nitrogen atoms possesses the best lysosome-localized ability. And DNS-C as a universal tracker exhibits excellent ability for lysosome labeling in different cell lines with high overlap coefficients (>= 0.90). Different from a commercially available LysoTracker, the Stokes shift of DNS-C up to 240 nm (lambda(ex/em) = 330/570 nm), is much larger than that of LysoTracker similar to 20 nm (lambda(ex/em) = 573/595 nm). More importantly, the fluorescence of DNS-C keeps still high brightness after a time-lapsed imaging for 40 min in living cells, implying its remarkable photostability for long-term tracking. In addition, DNS-C can also clearly image the autolysosome, a critical subcellular compartment, forming by the fusion of lysosome with autophagosome in autophagy. These results suggest the promising utility of our probe as a powerful tool to real-time trace physiological processes of lysosomes.
资助项目	National Natural Science Foundation of China[61705239] ; National Natural Science Foundation of China[21605145] ; National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21806168] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21976183] ; Hefei Physical Science Center Foundation[2017FXZY001]
WOS关键词	AUTOPHAGY ; PH ; TRACKING ; ENZYMES ; CARBON ; ACID
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000536207600014
资助机构	National Natural Science Foundation of China ; Hefei Physical Science Center Foundation
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103133]
专题	中国科学院合肥物质科学研究院
通讯作者	Han, Guangmei; Wang, Zhenyang
作者单位	1.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 3.Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China 4.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China 5.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Peoples R China
推荐引用方式 GB/T 7714	Yang, Linlin,Zhao, Jun,Wang, Jianping,et al. An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome[J]. TALANTA,2020,216.
APA	Yang, Linlin.,Zhao, Jun.,Wang, Jianping.,Han, Guangmei.,Liu, Bianhua.,...&Zhang, Zhongping.(2020).An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome.TALANTA,216.
MLA	Yang, Linlin,et al."An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome".TALANTA 216(2020).