Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis

doi:10.1631/jzus.B1900584

	Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis
	Qiao, En-qi; Yang, Hong-Jian; Zhang, Xi-ping
刊名	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
	2020-06-01
卷号	21
关键词	R979 1
ISSN号	1673-1581
DOI	10.1631/jzus.B1900584
通讯作者	Zhang, Xi-ping(zxp99688@sina.com)
英文摘要	The aim of this study was to identify some biomarkers for predicting lymph node metastasis and prognosis of human epidermal growth factor receptor 2 (Her-2)-positive breast cancer (BC). We analyzed correlations between microRNAs (miRNAs) and the prognosis of patients with BC based on data collected from The Cancer Genome Atlas (TCGA) database. The expression levels of miR-455, miR-143, and miR-99a were measured in clinical samples of Her-2-positive BC patients with different degrees of lymph node metastasis. We investigated the impacts of overexpressed miR-455 on the proliferation and invasiveness of MDA-MB-453 cells and measured its effects on the expression of long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) by quantitative real-time polymerase chain reaction (qRT-PCR). The expression of miR-455 was significantly and positively correlated to the prognosis and overall survival (OS) of the BC (P=0.028), according to TCGA information. The expression level of miR-455 was positively correlated with OS and relapse-free survival (RFS) of patients with Her-2- positive BC, and was negatively correlated with the number of metastatic lymph nodes (P0.05). Transwell assay suggested that MDA-MB-453 cells became much less invasive (P0.01) after being transfected with miR-455 mimics. During the qRT-PCR, the expression level ofMALAT1declined significantly after transfection (P0.01). Overexpressed miR-455 significantly inhibited the proliferation and migration of MDA-MB-453 cells and the expression ofMALAT1. We conclude that miR-455 may be a useful potential biomarker for forecasting lymph node metastasis and the prognosis of Her-2-positive BC patients. miR-455 may play an important role in lymph node metastasis of BC by interacting withMALAT1.
资助项目	Foundation for Key Platform Technological Project of Zhejiang Medical Science and Hygiene, China[2016ZDB003]
WOS关键词	SUPPRESSES CELL-PROLIFERATION ; TUMOR-SUPPRESSOR ; MICRORNAS ; MIGRATION ; INVASION ; EXPRESSION ; MALAT1 ; ANGIOGENESIS ; CHEMOTHERAPY ; APOPTOSIS
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Research & Experimental Medicine
语种	英语
出版者	ZHEJIANG UNIV
WOS记录号	WOS:000540161000007
资助机构	Foundation for Key Platform Technological Project of Zhejiang Medical Science and Hygiene, China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/103001]
专题	中国科学院合肥物质科学研究院
通讯作者	Zhang, Xi-ping
作者单位	Univ Chinese Acad Sci, Dept Breast Surg, Canc Hosp, Zhejiang Canc Hosp, Hangzhou 310022, Peoples R China
推荐引用方式 GB/T 7714	Qiao, En-qi,Yang, Hong-Jian,Zhang, Xi-ping. Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2020,21.
APA	Qiao, En-qi,Yang, Hong-Jian,&Zhang, Xi-ping.(2020).Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,21.
MLA	Qiao, En-qi,et al."Screening of miRNAs associated with lymph node metastasis in Her-2-positive breast cancer and their relationship with prognosis".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 21(2020).