Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese

doi:10.1089/gtmb.2019.0269

	Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese
	Yu, Aijun 5,6; Zhang, Jiejie 5,6; Mei, Yuxian 4; Zhong, Hai 3; Chen, Shasha 2; Song, Qian 1
刊名	GENETIC TESTING AND MOLECULAR BIOMARKERS
	2020-07-01
卷号	24
关键词	cervical cancer PTEN single nucleotide polymorphism miRNA
ISSN号	1945-0265
DOI	10.1089/gtmb.2019.0269
通讯作者	Chen, Shasha(CSSCSS724724@163.com) ; Song, Qian(happysongqian@126.com)
英文摘要	Objective:To analyze the association between a single nucleotide polymorphism (SNP) of the 3 ' untranslated region (UTR) of the phosphatase and tensin homolog (PTEN)gene, that is within a microRNA (miRNA) binding site, and the risk of Chinese Han cervical cancer. Methods:A case-control study was carried out to analyze the genotype of thePTENrs34140758 locus in 210 surgically treated, Han Chinese, cervical cancer patients and 210 healthy controls. The levels of the miRNAs hsa-miR-586 and hsa-miR-622 and thePTENmRNA were analyzed by real-time reverse transcription-quantitative polymerase chain reaction in the cancerous and adjacent normal tissues from all cases. HeLa cells were transfected with the miRNAs, hsa-miR-586 and hsa-miR-622, to analyze their effects on PTEN gene expression. Results:After adjusting for age, body-mass index, alcohol consumption, smoking, and familial history of cancer, thePTENrs34140758 A allele carriers were 1.47 times more likely to suffer from cervical cancer than the C allele carriers (odds ratio [OR] = 1.47, 95% confidence interval [CI]: 1.17-1.72,p = 0.001). Both hsa-miR-586 and hsa-miR-622 were highly expressed in the cancerous tissues of the cervical cancer patients, whereasPTENexpression was low. HeLa cell transfection experiments showed that hsa-miR-586 and hsa-miR-622 inhibitedPTENgene expression. The results of a dual-luciferase reporter assay showed that thePTENgene is a target for both hsa-miR-586 and hsa-miR-622. Conclusion:ThePTEN3 ' UTR rs34140758 locus SNP is associated with the risk of cervical cancer in the Han Chinese population. The molecular mechanism may be that the rs34140758 SNP affects the regulation ofPTENgene expression through interaction with the hsa-miR-586 and hsa-miR-622 miRNAs.
资助项目	Zhejiang Provincial Medicine Health Science and Technology Program[2019ZA021]
WOS关键词	PTEN ; EXPRESSION ; MIGRATION ; DIAGNOSIS ; INVASION ; MIR-622
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	MARY ANN LIEBERT, INC
WOS记录号	WOS:000541879100001
资助机构	Zhejiang Provincial Medicine Health Science and Technology Program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/71350]
专题	中国科学院合肥物质科学研究院
通讯作者	Chen, Shasha; Song, Qian
作者单位	1.Taizhou Canc Hosp, Dept Gynecol & Obstet, 50 Zhenxin Rd, Wenling 317502, Zhejiang, Peoples R China 2.Taizhou Canc Hosp, Dept Tradit Chinese Med, 50 Zhenxin Rd, Wenling 317502, Zhejiang, Peoples R China 3.Second Hosp Yinzhou Dist, Dept Cardiothorac Surg, Ningbo, Zhejiang, Peoples R China 4.Wenling Hosp Tradit Chinese Med, Dept Urol, Wenling, Zhejiang, Peoples R China 5.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China 6.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Gynecol Oncol, Hangzhou, Peoples R China
推荐引用方式 GB/T 7714	Yu, Aijun,Zhang, Jiejie,Mei, Yuxian,et al. Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese[J]. GENETIC TESTING AND MOLECULAR BIOMARKERS,2020,24.
APA	Yu, Aijun,Zhang, Jiejie,Mei, Yuxian,Zhong, Hai,Chen, Shasha,&Song, Qian.(2020).Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese.GENETIC TESTING AND MOLECULAR BIOMARKERS,24.
MLA	Yu, Aijun,et al."Correlation Between Single Nucleotide Polymorphisms of an miRNA Binding Site in the 3 ' UTR ofPTENand Risk of Cervical Cancer Among the Han Chinese".GENETIC TESTING AND MOLECULAR BIOMARKERS 24(2020).