Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer

	Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer
刊名	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
	2017
卷号	18
关键词	DNA-BASE 5-HYDROXYMETHYLCYTOSINE GENE-EXPRESSION COLON-CANCER LUNG-CANCER HYPOMETHYLATION METHYLTRANSFERASES HYPERMETHYLATION QUANTIFICATION DEMETHYLATION METHYLATION 5-Hydroxymethylcytosine (5hmC) 5-Methylcytosine (5mC) Prognosis Colorectal cancer
ISSN号	1673-1581
其他题名	Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer *
英文摘要	Aberrant DNA methylation has raised widespread attention in tumorigenesis. In this study, we aimed to investigate the changes of global DNA methylation and hydroxymethylation from normal to tumor tissues in colorectal cancer (CRC) and their association with the prognosis. The levels of genomic 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) in cancerous tissues were significantly lower than those in corresponding adjacent normal tissues. The genomic levels of 5mC were significantly positively correlated with 5hmC in normal and cancerous tissues (all P < 0.05). The ratio of 5mC in cancerous tissues to matched normal tissues (C/N-5mC) was also significantly positively correlated with the ratio of 5hmC in cancerous tissues to matched normal tissues (C/N-5hmC) (P=0.01). The 5mC levels and C/N-5mC ratios decreased with age (all P < 0.05). Higher 5mC and 5hmC levels were found in rectal than in colon tissues (all P < 0.05). High levels of 5mC in cancerous tissues and high C/N-5hmC ratios were each associated with lymph node metastasis (all P < 0.05). Survival analysis indicated that the C/N-5mC ratio (P=0.04) is an independent protective factor for overall survival. The data showed that patients with a combination of high C/N-5hmC and low C/N-5mC ratios tended to have a worse prognosis (P < 0.01). Our findings showed that the C/N-5mC ratio may be an independent prognostic factor for CRC outcome. Patients with both a high C/N-5hmC ratio and a low C/N-5mC ratio exhibited the worst survival, suggesting that 5mC and 5hmC can be used as critical markers in tumorigenesis and prognosis estimation.
资助项目	[111 Project] ; [National High-Tech R&D Program (863) of China] ; [Fundamental Research Funds for the Central Universities] ; [Zhejiang Provincial Program for the Cultivation of High-Level Innovative Health Talents]
语种	英语
CSCD记录号	CSCD:6072070
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/51718]
专题	中国科学院合肥物质科学研究院
推荐引用方式 GB/T 7714	. Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2017,18.
APA	(2017).Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,18.
MLA	"Global changes of 5-hydroxymethylcytosine and 5-methylcytosine from normal to tumor tissues are associated with carcinogenesis and prognosis in colorectal cancer".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 18(2017).