Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak

	Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak
	Xu, Shang-Rang; Wei, Peng; Yang, Qi-Lin; Jia, Gong-Xue; Ma, Shi-Ke; Yang, Qi-En; Jun, Zhang; Zhang, Rui-Na
刊名	THERIOGENOLOGY
	2020
卷号	147
关键词	Yak Ovary Seasonal reproduction Estrogen Transcriptome Follicle
英文摘要	Domestic yaks are the most important livestock species on the Qinghai-Tibetan Plateau. Adult female yaks normally breed in warm season and enter anestrous in cold season. Currently, how the ovarian activity is regulated at the molecular level remains to be determined. This study was conducted to investigate follicular development and gene expression patterns of yak ovarian tissues in the warm and cold seasons. Dynamics of follicular development was evaluated based on histological analyses and global gene expression was examined by using RNA-sequencing (RNA-seq) technology. Firstly, we found that follicle development of yak cows in cold season was different from that in warm season. Interestingly, ovaries collected from yaks in cold season contained a significant higher number of antral follicles and some of these follicles showed signs of polycystic structure, indicating abnormal granulosa cell function. RNA-seq analyses of ovarian tissues from non-pregnant adult yaks in cold and warm season revealed that a list of 320 transcripts were differentially expressed, specifically, 79 were up-regulated and 241 were down-regulated in the ovaries from yaks during the cold season. Further analysis demonstrated that transcripts associated with estrogen secretion and metabolism signaling pathway were altered, including FST, CYP1A1, PIK3R1 and PIK3R2. This study showed histological features of follicle development and revealed candidate genes that may have important roles in regulating ovarian activities in the yak seasonal reproduction. (C) 2020 Elsevier Inc. All rights reserved.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60356]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Xu, Shang-Rang,Wei, Peng,Yang, Qi-Lin,et al. Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak[J]. THERIOGENOLOGY,2020,147.
APA	Xu, Shang-Rang.,Wei, Peng.,Yang, Qi-Lin.,Jia, Gong-Xue.,Ma, Shi-Ke.,...&Zhang, Rui-Na.(2020).Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak.THERIOGENOLOGY,147.
MLA	Xu, Shang-Rang,et al."Transcriptome analysis revealed key signaling networks regulating ovarian activities in the domestic yak".THERIOGENOLOGY 147(2020).