Copper nanoclusters@Al(3+)complexes with strong and stable aggregation-induced emission for application in enzymatic determination of urea

	Copper nanoclusters@Al(3+)complexes with strong and stable aggregation-induced emission for application in enzymatic determination of urea
	Qu, Fei; Wang, Bingjie; Li, Kexin; You, Jinmao; Han, Wenli
刊名	MICROCHIMICA ACTA
	2020
卷号	187 期号:8
关键词	Enzymatic determination Bioimaging Urease Fluorescence Glutathione Human urine
英文摘要	The aggregates of glutathione-capped CuNCs induced by Al3+(named CuNCs@Al(3+)complexes) show a stable aggregation-induced emission (AIE) for about 1 month. Their fluorescence maintains a high level in the pH range 4.0 to 7.0, while it quenches as pH increases from 7.0 to 7.7 or decreases from 4.0 to 3.0. Under urease-catalyzed hydrolysis, urea produces ammonia, which can be further hydrolyzed to yield OH-. This leads to a pH increase of the immediate environment. Hence, the CuNCs@Al(3+)complexes are a suitable probe to determine urea. The fluorescence of CuNCs@Al(3+)complexes quenches linearly at 585 nm with the excitation wavelength at 340 nm when the concentration of urea increases from 20 to 150 mu M. The limit of detection (LOD) of urea is 5.86 mu M. This sensitivity is superior to other reported works due to the narrow pH response range of CuNCs@Al(3+)complexes. This method has been successfully applied for measuring urea in human urine samples with satisfactory recoveries.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/60293]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Qu, Fei,Wang, Bingjie,Li, Kexin,et al. Copper nanoclusters@Al(3+)complexes with strong and stable aggregation-induced emission for application in enzymatic determination of urea[J]. MICROCHIMICA ACTA,2020,187(8).
APA	Qu, Fei,Wang, Bingjie,Li, Kexin,You, Jinmao,&Han, Wenli.(2020).Copper nanoclusters@Al(3+)complexes with strong and stable aggregation-induced emission for application in enzymatic determination of urea.MICROCHIMICA ACTA,187(8).
MLA	Qu, Fei,et al."Copper nanoclusters@Al(3+)complexes with strong and stable aggregation-induced emission for application in enzymatic determination of urea".MICROCHIMICA ACTA 187.8(2020).