Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography

doi:10.1039/d1bm00174d

	Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography
	Luo CH(罗春花)3,4; Lv DY(吕东媛)2,3,4; Zheng L(郑鲁)2,3,4; Zhang F(张帆)2,3,4; Zhang X(张潇)2,3,4; Lv SQ(吕守芹)2,3,4; Zhang C(张晨)3,4; Jia Xiaohua 1; Shu XY(舒芯钰)2,3,4; Li PW(李培文)3,4
刊名	BIOMATERIALS SCIENCE
	2021-04-20
页码	15
ISSN号	2047-4830
DOI	10.1039/d1bm00174d
通讯作者	Long, Mian(mlong@imech.ac.cn)
英文摘要	Mechanical or physical cues are associated with the growth and differentiation of embryonic stem cells (ESCs). While the substrate stiffness or topography independently affects the differentiation of ESCs, their cooperative regulation on lineage-specific differentiation remains largely unknown. Here, four topographical configurations on stiff or soft polyacrylamide hydrogel were combined to direct hepatic differentiation of human H1 cells via a four-stage protocol, and the coupled impacts of stiffness and topography were quantified at distinct stages. Data indicated that the substrate stiffness is dominant in stemness maintenance on stiff gel and hepatic differentiation on soft gel while substrate topography assists the differentiation of hepatocyte-like cells in positive correlation with the circularity of H1 clones initially formed on the substrate. The differentiated cells exhibited liver-specific functions such as maintaining the capacities of CYP450 metabolism, glycogen synthesis, ICG engulfment, and repairing liver injury in CCl4-treated mice. These results implied that the coupling of substrate stiffness and topography, combined with the biochemical signals, is favorable to improve the efficiency and functionality of hepatic differentiation of human ESCs.
分类号	二类/Q1
资助项目	National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870931] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31470907] ; National Natural Science Foundation of China[81703012] ; Chinese Science Academy[QYZDJ-SSW-JSC018] ; Chinese Science Academy[GJJSTU20190005] ; Chinese Science Academy[XDB22040101]
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000641302300001
资助机构	National Natural Science Foundation of China ; Chinese Science Academy
其他责任者	Long, Mian
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/86479]
专题	力学研究所_国家微重力实验室
作者单位	1.Chinese Acad Sci, Inst Automat, CAS Key Lab Mol Imaging, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China; 3.Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China; 4.Chinese Acad Sci, Inst Mech, Ctr Biomech & Bioengn, Key Lab Micrograv,Natl Micrograv Lab, Beijing 100190, Peoples R China;
推荐引用方式 GB/T 7714	Luo CH,Lv DY,Zheng L,et al. Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography[J]. BIOMATERIALS SCIENCE,2021:15.
APA	罗春花.,吕东媛.,郑鲁.,张帆.,张潇.,...&龙勉.(2021).Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography.BIOMATERIALS SCIENCE,15.
MLA	罗春花,et al."Hepatic differentiation of human embryonic stem cells by coupling substrate stiffness and microtopography".BIOMATERIALS SCIENCE (2021):15.