Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands | |
Shu XY(舒芯钰)1,2; Li N(李宁); Huang DD(黄丹丹)1,2; Zhang Y(章燕)1,2; Lv SQ(吕守芹)1,2; Long M(龙勉)1,2 | |
刊名 | EXPERIMENTAL CELL RESEARCH |
2020-02-15 | |
卷号 | 387期号:2页码:7 |
关键词 | beta(2) integrin Ligands Calcium transients Bond strength Mechanotransduction Shear stress |
ISSN号 | 0014-4827 |
DOI | 10.1016/j.yexcr.2019.111807 |
英文摘要 | Lymphocyte function-associated antigen-1 (LFA-1) and macrophage-1 antigen (Mac-1) are key adhesion receptors to mediate neutrophil (PMN) recruitment and intracellular calcium (Ca2+) signaling. Binding of LFA-1 and Mac-1 to their ligands is essential in triggering Ca2+ transients and activating Ca2+-dependent kinases involved in cytoskeletal remodeling and migratory function. While mechanical forces are critical in regulating integrin-mediated Ca2+ transients, it is still unclear how the bond strength of beta(2)-integrin-ligand pair affects Ca2+ responses. Here three typical ligands with known mechanical features with LFA-1 and Mac-1 in our previous work were adopted to quantify their capabilities in inducing Ca2+ transients in adherent PMNs under shear flow. Data indicated that LFA-1 dominates Ca2+ transients in PMNs on intercellular adhesive molecule 1 (ICAM-1) and junctional adhesion molecule-A (JAM -A), while Mac-1 mediates Ca2+ transients induced by receptor for advanced glycation end products (RAGE), consistent with their corresponding bond strengths. These results link beta(2) integrin-ligand bond strength with Ca2+ transients in PMNs, suggesting high bond strength gives rise to strong Ca2+ response especially under physiological-like shear flow. The outcomes provide a new insight in understanding the mechanical regulatory mechanisms of PMN recruitment. |
分类号 | 二类 |
资助项目 | National Natural Science Foundation of China[91642203] ; National Natural Science Foundation of China[31661143044] ; National Natural Science Foundation of China[31627804] ; National Natural Science Foundation of China[31870930] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[QYZDJ-SSW-JSC018] ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences[XDB22040101] |
WOS关键词 | JUNCTIONAL ADHESION MOLECULE ; FORCE ; ACTIVATION ; BINDING ; STRETCH ; ACCUMULATION ; PATHWAY ; INFLUX ; LFA-1 ; CELLS |
WOS研究方向 | Oncology ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000512482300015 |
资助机构 | National Natural Science Foundation of China ; Strategic Priority Research Program and Frontier Science Key Project of Chinese Academy of Sciences |
其他责任者 | Long, Mian |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/81592] |
专题 | 力学研究所_国家微重力实验室 |
作者单位 | 1.Chinese Acad Sci, Ctr Biomech & Bioengn, Inst Mech, Key Lab Micrograv,Natl Micrograv Lab,Beijing Key, Beijing 100190, Peoples R China; 2.Univ Chinese Acad Sci, Sch Engn Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Shu XY,Li N,Huang DD,et al. Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands[J]. EXPERIMENTAL CELL RESEARCH,2020,387(2):7. |
APA | Shu XY,Li N,Huang DD,Zhang Y,Lv SQ,&Long M.(2020).Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands.EXPERIMENTAL CELL RESEARCH,387(2),7. |
MLA | Shu XY,et al."Mechanical strength determines Ca2+ transients triggered by the engagement of beta(2) integrins to their ligands".EXPERIMENTAL CELL RESEARCH 387.2(2020):7. |
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