Compression Generated by a 3D Supracellular Actomyosin Cortex Promotes Embryonic Stem Cell Colony Growth and Expression of Nanog and Oct4 | |
Du J; Fan YL; Guo Z; Wang YG; Zheng X(郑旭); Huang C; Liang BH; Gao LY; Cao YP; Chen YP | |
刊名 | CELL SYSTEMS |
2019-08-28 | |
卷号 | 9期号:2页码:214-+ |
ISSN号 | 2405-4712 |
DOI | 10.1016/j.cels.2019.05.008 |
英文摘要 | Mechanical factors play critical roles in mammalian development. Here, we report that colony-growing mouse embryonic stem cells (mESCs) generate significant tension on the colony surface through the contraction of a three-dimensional supracellular actomyosin cortex (3D-SAC). Disruption of the 3D-SAC, whose organization is dependent on the Rho/Rho-associated kinase (ROCK) signals and E-cadherin, results in mESC colony destruction. Reciprocally, compression force, which is generated by the 3D-SAC, promotes colony growth and expression of Nanog and Oct4 in mESCs and blastocyst development of mouse embryos. These findings suggest that autonomous cell forces regulate embryonic stem cells fate determination and provide insight regarding the biomechanical regulation of embryonic development. |
分类号 | 一类 |
资助项目 | National Key R&D Program of China[2017YFA0506500] ; National Natural Science Foundation of China[31370018] ; National Natural Science Foundation of China[11620101001] ; Fundamental Research Funds for the Central Universities[ZG140S1971] ; NSF[DMR-1708729] ; Harvard MRSEC[DMR-1420570] |
WOS关键词 | RHO-ASSOCIATED KINASE ; MYOSIN ; PHOSPHORYLATION ; MECHANICS ; STIFFNESS ; FRET |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
语种 | 英语 |
WOS记录号 | WOS:000483697600009 |
资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; NSF ; Harvard MRSEC |
其他责任者 | Du, Jing ; Weitz, David A. ; Feng, Xiqiao |
内容类型 | 期刊论文 |
源URL | [http://dspace.imech.ac.cn/handle/311007/79937] |
专题 | 力学研究所_非线性力学国家重点实验室 |
推荐引用方式 GB/T 7714 | Du J,Fan YL,Guo Z,et al. Compression Generated by a 3D Supracellular Actomyosin Cortex Promotes Embryonic Stem Cell Colony Growth and Expression of Nanog and Oct4[J]. CELL SYSTEMS,2019,9(2):214-+. |
APA | Du J.,Fan YL.,Guo Z.,Wang YG.,Zheng X.,...&Feng XQ.(2019).Compression Generated by a 3D Supracellular Actomyosin Cortex Promotes Embryonic Stem Cell Colony Growth and Expression of Nanog and Oct4.CELL SYSTEMS,9(2),214-+. |
MLA | Du J,et al."Compression Generated by a 3D Supracellular Actomyosin Cortex Promotes Embryonic Stem Cell Colony Growth and Expression of Nanog and Oct4".CELL SYSTEMS 9.2(2019):214-+. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论