梯度洗脱高效液相色谱法快速检测厌氧菌代谢物中的有机酸

	梯度洗脱高效液相色谱法快速检测厌氧菌代谢物中的有机酸
	刘晨明; 曹宏斌; 曹俊雅; 李玉平; 张懿
刊名	分析化学
	2006
卷号	34 期号:9 页码:1231
ISSN号	0253-3820
英文摘要	A high performance liquid chromatographic method was set up for fast detecting formic acid, lactic acid, acetic acid, succinic acid and propionic acid. The mobile phase was acetonitrile and KH2 PO4 (0.02 mol/L, pH 2.8), flow rate and the ratio of organic solvent were controlled by a gradient elution programme with quaternary pump, detection wavelength was 215 nm, and column temperature was 30 degrees C. The method could fast and effectively separate and detect the organic acids in anaerobe medium. The analyzed time was 4 minutes. The RSD was 0.3% - 1.3%, recovery was 95.0% - 100.8%, all the correlation coefficients (r) were no less than 0.9998. The method was precise and Accurate, and could be used for analyzing the organic acids in anaerobe medium.
语种	英语
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/32500]
专题	中国科学院过程工程研究所
作者单位	中国科学院过程工程研究所
推荐引用方式 GB/T 7714	刘晨明,曹宏斌,曹俊雅,等. 梯度洗脱高效液相色谱法快速检测厌氧菌代谢物中的有机酸[J]. 分析化学,2006,34(9):1231.
APA	刘晨明,曹宏斌,曹俊雅,李玉平,&张懿.(2006).梯度洗脱高效液相色谱法快速检测厌氧菌代谢物中的有机酸.分析化学,34(9),1231.
MLA	刘晨明,et al."梯度洗脱高效液相色谱法快速检测厌氧菌代谢物中的有机酸".分析化学 34.9(2006):1231.