Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice

doi:10.1016/j.mrgentox.2012.07.001

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	Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice
	Wu, Mengjun 1,2; Xing, Guozhen 1; Qi, Xinming1 ; Feng, Chenchen 1,2; Liu, Mingxia 1,2; Gong, Likun1 ; Luan, Yang 1; Ren, Jin1
刊名	MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
	2012-10-09
卷号	748 期号:1-2 页码:65-69
关键词	Arecoline gpt delta transgenic mouse Mutagenesis
ISSN号	1383-5718
DOI	10.1016/j.mrgentox.2012.07.001
文献子类	Article
英文摘要	Chewing the areca nut is carcinogenic to humans. Arecoline, a major alkaloid in areca nut, is suspected to be a carcinogenic component. It has been shown to have genotoxic potential in various in vitro systems; but information on its in vivo genotoxicity is limited. To investigate the organ-specific mutagenic potential of arecoline, we employed gpt delta transgenic mice to analyze the mutagenicity of arecoline in the oral tissues and liver. Male gpt delta mice were given arecoline hydrobromide in drinking water at 300 and 700 mu g/mL for 6 weeks. 4-Nitroquinoline-1 (4-NQO) was used as a positive control. Two weeks after the last treatment, mutation frequencies in the oral tissues (a mixture of gingival, buccal, pharyngeal and sublingual tissue) and liver were detected and mutation spectra were analyzed. There were no statistically significant differences in the average mutation frequencies between arecoline-treated and untreated groups in both the oral tissues and liver. However, in the oral tissues, one mouse in arecoline-300 mu g/mL group and two mice in arecoline-700 mu g/mL group showed more than 2.5-fold higher mutation frequencies than the untreated group; they also exhibited unique mutation spectra compared to spontaneous mutation types. In these three mice, all mutations occurred at G:C sites, where G:C -> T:A transversions were most frequent, followed by G:C -> A:T transitions and G:C -> C:G transversions. The main type of spontaneous mutation in both the oral tissues and liver was G:C -> A:T transition. These results suggest that arecoline poses a mutagenic hazard in the oral tissues of gpt delta transgenic mice. (c) 2012 Elsevier B.V. All rights reserved.
资助项目	Key Projects of National Science and Technology Pillar Program[2012ZX09301001-006] ; Key Projects of National Science and Technology Pillar Program[2012zx09302003] ; Public Service Platform Project of Shanghai Science and Technology Committee[11DZ2292500]
WOS关键词	HUMAN GINGIVAL FIBROBLASTS ; ORAL SUBMUCOUS FIBROSIS ; BUCCAL EPITHELIAL-CELLS ; BETEL QUID CHEWERS ; ARECA NUT EXTRACT ; DNA-DAMAGE ; IN-VITRO ; OXIDATIVE-STRESS ; CARCINOGENESIS ; INGREDIENTS
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000308515300011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277912]
专题	药物安全性评价中心
通讯作者	Luan, Yang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab New Drug Res, Ctr Drug Safety & Evaluat Res, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Grad Sch, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wu, Mengjun,Xing, Guozhen,Qi, Xinming,et al. Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2012,748(1-2):65-69.
APA	Wu, Mengjun.,Xing, Guozhen.,Qi, Xinming.,Feng, Chenchen.,Liu, Mingxia.,...&Ren, Jin.(2012).Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,748(1-2),65-69.
MLA	Wu, Mengjun,et al."Assessment of the mutagenic potential of arecoline in gpt delta transgenic mice".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 748.1-2(2012):65-69.