Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies

doi:10.1186/1471-2164-15-488

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	Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies
	Zhao, Botao 2; Yang, Dehua3,4 ; Jiang, Jing 1; Li, Jinsong 1; Fan, Chunsun 5; Huang, Menggui 6; Fan, Yi 6; Jin, Yan 7; Jin, Youxin 2
刊名	BMC GENOMICS
	2014-06-18
卷号	15
ISSN号	1471-2164
DOI	10.1186/1471-2164-15-488
文献子类	Article
英文摘要	Background: Reprogrammed cells, including induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cells (NT-ESCs), are similar in many respects to natural embryonic stem cells (ESCs). However, previous studies have demonstrated that iPSCs retain a gene expression signature that is unique from that of ESCs, including differences in microRNA (miRNA) expression, while NT-ESCs are more faithfully reprogrammed cells and have better developmental potential compared with iPSCs. Results: We focused on miRNA expression and explored the difference between ESCs and reprogrammed cells, especially ESCs and NT-ESCs. We also compared the distinct expression patterns among iPSCs, NT-ESCs and NT-iPSCs. The results demonstrated that reprogrammed cells (iPSCs and NT-ESCs) have unique miRNA expression patterns compared with ESCs. The comparison of differently reprogrammed cells (NT-ESCs, NT-iPSCs and iPSCs) suggests that several miRNAs have key roles in the distinct developmental potential of reprogrammed cells. Conclusions: Our data suggest that miRNAs play a part in the difference between ESCs and reprogrammed cells, as well as between MEFs and pluripotent cells. The variation of miRNA expression in reprogrammed cells derived using different reprogramming strategies suggests different characteristics induced by nuclear transfer and iPSC generation, as well as different developmental potential among NT-ESCs, iPSCs and NT-iPSCs.
资助项目	National Key Research and Development Program of China[2011CB811304] ; National Natural Science Foundation of China[31170750] ; National Natural Science Foundation for Young Scholar[31100570] ; Shanghai Municipal Education Commission[12YZ032] ; Shanghai University[00000000] ; Natural Science Foundation of Shanghai City[12ZR1406900] ; State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Institutes for Biological Sciences, Chinese Academy of Sciences[00000000]
WOS关键词	NUCLEAR TRANSFER ; HUMAN FIBROBLASTS ; MOUSE ; MICRORNAS ; MIR-302 ; IPS ; BLASTOCYSTS ; INDUCTION ; MIRBASE ; TARGETS
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000338246200002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277019]
专题	国家新药筛选中心
通讯作者	Jin, Youxin
作者单位	1.Chinese Acad Sci, Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China; 2.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China; 5.Qidong Liver Canc Inst, Dept Etiol, Qidong 226200, Jiangsu, Peoples R China; 6.Univ Penn, Dept Radiat Oncol, Philadelphia, PA 19104 USA; 7.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Shenzhen 518055, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Botao,Yang, Dehua,Jiang, Jing,et al. Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies[J]. BMC GENOMICS,2014,15.
APA	Zhao, Botao.,Yang, Dehua.,Jiang, Jing.,Li, Jinsong.,Fan, Chunsun.,...&Jin, Youxin.(2014).Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies.BMC GENOMICS,15.
MLA	Zhao, Botao,et al."Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies".BMC GENOMICS 15(2014).