Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery

doi:10.1039/c6tb02788a

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	Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery
	Wang, Xuefang 4,5; Wu, Zhitao 1; Li, Jianbo 4,5; Pan, Guoyu1 ; Shi, Donglu 2,3; Ren, Jie 3,4,5
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2017-01-14
卷号	5 期号:2 页码:296-306
ISSN号	2050-750X
DOI	10.1039/c6tb02788a
文献子类	Article
英文摘要	A thermo-responsive PCL/PEG analogue copolymer (PCL-[b-P(MEO(2)MA-co-OEGMA)](2)) with a lower critical solution temperature (LCST) of 40.4 degrees C at an MEO(2)MA/OEGMA molar ratio of 87 : 13 was designed and synthesized. The copolymer was subsequently labeled by coupling with fluorescein isothiocyanate (FITC). Thermo-responsive magnetic PCL-[b-P(MEO(2)MA-co-OEGMA)](2)/Mn0.6Zn0.4Fe2O4 (MZF) complex micelles were prepared by a self-assembly method. Doxorubicin (DOX) was loaded into the magnetic complex micelles as a model drug, and the DOX-MZF-micelles showed well-controlled thermo-responsive release both at externally fixed temperatures and in the presence of an alternating magnetic field (AMF). Both the blank polymer micelles and the magnetic complex micelles exhibited excellent stability in normal saline and serum. Based on the detection of the FITC fluorescence signal, the micelles were found to be effectively labeled by FITC. Furthermore, the biological toxicity of micelles was studied in vitro and in vivo. In vitro toxicity studies to evaluate cell viability and cell toxicity were performed by employing WST-1 and LDH release assays using HL7702 cells, respectively. In vivo biotoxicity studies were conducted in ICR mice through a series of tests: general conditions, body weight shifts, serum biochemistry profiles, and organ coefficient tests. All the biological toxicity results obtained from the blank polymer micelles and the magnetic complex micelles indicated their good biocompatibility and nontoxicity. The in vivo biodistribution studies of the FITC-labeled magnetic complex micelles were performed in the ICR mice. The copolymer was cleared by the kidney and spleen, while the MZF nanoparticles were cleared by the liver in time, causing no adverse effects on organisms. The thermo-responsive magnetic complex micelles were shown to be an ideal nanocarrier for anticancer drug delivery in terms of controlled release, stability, biocompatibility and safety.
资助项目	National High Technology Research and Development Program of China[2013AA032202] ; Fundamental Research Funds for the Central Universities[00000000] ; Open Funds for Characterization of Tongji University[00000000]
WOS关键词	THERMO-CHEMOTHERAPY ; CANCER-CELLS ; NANOPARTICLES ; DOXORUBICIN ; HYPERTHERMIA ; POLYMERS ; RELEASE ; TEMPERATURE ; THERAPY ; SYSTEM
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000392418800011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275676]
专题	药物安全性评价中心
通讯作者	Li, Jianbo; Ren, Jie
作者单位	1.Chinese Acad Sci, Ctr Drug Safety Evaluat & Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Univ Cincinnati, Coll Engn & Appl Sci, Dept Mech & Mat Engn, Mat Sci & Engn Program, Cincinnati, OH 45221 USA 3.Tongji Univ, Sch Med, Shanghai East Hosp, Inst Biomed Engn & Nano Sci,Inst Translat Nanomed, Shanghai 200120, Peoples R China; 4.Tongji Univ, Sch Mat Sci & Engn, Inst Nano & Biopolymer Mat, 4800 Caoan Rd, Shanghai 201804, Peoples R China; 5.Tongji Univ, Key Lab Adv Civil Engn Mat, Minist Educ, Shanghai 201804, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Xuefang,Wu, Zhitao,Li, Jianbo,et al. Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(2):296-306.
APA	Wang, Xuefang,Wu, Zhitao,Li, Jianbo,Pan, Guoyu,Shi, Donglu,&Ren, Jie.(2017).Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery.JOURNAL OF MATERIALS CHEMISTRY B,5(2),296-306.
MLA	Wang, Xuefang,et al."Preparation, characterization, biotoxicity, and biodistribution of thermo-responsive magnetic complex micelles formed by Mn0.6Zn0.4Fe2O4 and a PCL/PEG analogue copolymer for controlled drug delivery".JOURNAL OF MATERIALS CHEMISTRY B 5.2(2017):296-306.