Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study

doi:10.3390/molecules23113020

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	Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study
	Wei, Wenlong 1,2; Wang, Xia 2; Hou, Jinjun2 ; Yao, Changliang 2; Feng, Zijin 2; Zhang, Jianqing 2; Han, Sumei 2; Deng, Yanping 2; Huang, Yong 2; Wu, Wanying2
刊名	MOLECULES
	2018-11
卷号	23 期号:11
关键词	mass spectrometry fingerprint Venenum bufonis quality control chemometrics analysis
ISSN号	1420-3049
DOI	10.3390/molecules23113020
文献子类	Article
英文摘要	The mass spectrometry (MS) has been widely used for profiling chemical components of traditional Chinese medicine (TCM). However, there are few studies reporting quality control of TCM based on mass spectrometry fingerprint (MSF) due to its complicated operation and high cost. The aim of this study was to extend the application of MSF for quality evaluation of TCM. In this study, an MSF based on single quadrupole mass spectrometry method was established, and was successfully used for the quality control of Venenum bufonis (VB), a famous TCM which was used clinically for cancer treatment in China. The results showed that the superiority of MSF for more chemical information exposure and the finding of more potential chemical markers (eight versus four) compared with the traditional photo-diode array (a kind of ultra violet detector, PDA). Besides, the performance of MSF was also validated by similarity and principle component analysis (PCA) of MS data acquired on two other mass spectrometry (low-resolution, triple quadrupole, QQQ, and high-resolution, quadruple time-of-flight, Q-TOF), showing high consistency with QQQ and Q-TOF, but robustness with few parameters' settings. Based on our study, MSF could be widely applied for the quality control of TCM.
资助项目	National Natural Science Foundation of China[81530095] ; National Natural Science Foundation of China[81673591] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA12020348] ; National Standardization of Traditional Chinese Medicine Project[ZYBZH-K-LN-01] ; Science and Technology Commission Foundation of Shanghai[15DZ0502800]
WOS关键词	QUALITY CONSISTENCY ; CHROMATOGRAPHY ; GINSENOSIDES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000451641900292
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279504]
专题	上海中药现代化研究中心
通讯作者	Wu, Wanying; Guo, Dean
作者单位	1.China Pharmaceut Univ, Sch Pharm, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wei, Wenlong,Wang, Xia,Hou, Jinjun,et al. Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study[J]. MOLECULES,2018,23(11).
APA	Wei, Wenlong.,Wang, Xia.,Hou, Jinjun.,Yao, Changliang.,Feng, Zijin.,...&Guo, Dean.(2018).Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study.MOLECULES,23(11).
MLA	Wei, Wenlong,et al."Implementation of a Single Quadrupole Mass Spectrometer for Fingerprint Analysis: Venenum bufonis as a Case Study".MOLECULES 23.11(2018).