Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration

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	Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration
	Jiang, Weixing 1; Liu, Shuang 1,2; Wu, Bin 2; Huang, Chenggang2
刊名	ASIAN JOURNAL OF CHEMISTRY
	2011-04
卷号	23 期号:4 页码:1643-1647
关键词	Ablmosechus manihot HPLC-MS(n) Metabolites Urine
ISSN号	0970-7077
文献子类	Article
英文摘要	A highly specific and sensitive method using high performance liquid chromatography-electrospray ionization (ESI) tandem ion trap mass spectrometry(HPLC-ESI-MS(n)) was developed to study on A. manihot and its metabolites in rat urine. The gradient elution reversed phase HPLC system using water of 0.5 % formic acid and acetonitrile as gradient Solvent mixtures was able to separate all metabolites of Ablmosechus manihot. The A. manihot were negative identified by the protonated molecules [M-H](-) and further characterized by tandem mass spectrometric analysis with each it giving a characteristic collision induced dissociation product ion spectrum. Furthermore, 2 prototype constituents and 7 metabolites in urine were detected and elucidated. This convenient HPLC-MS(n) method could be used to identify the chemical components of extract of A. manihot as well as their metabolites and to reveal their possible metabolic mechanism of action in vivo.
资助项目	National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09301-001] ; National Science & Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005]
WOS关键词	ABELMOSCHUS-MANIHOT ; ARRAY DETECTION ; HPLC ; FLAVONOLS ; FLOWERS ; EXTRACT ; PLASMA
WOS研究方向	Chemistry
语种	英语
出版者	ASIAN JOURNAL OF CHEMISTRY
WOS记录号	WOS:000288465100053
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278572]
专题	上海中药现代化研究中心
通讯作者	Wu, Bin
作者单位	1.Haerbin Univ Commerce, Haerbin 150076, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Weixing,Liu, Shuang,Wu, Bin,et al. Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration[J]. ASIAN JOURNAL OF CHEMISTRY,2011,23(4):1643-1647.
APA	Jiang, Weixing,Liu, Shuang,Wu, Bin,&Huang, Chenggang.(2011).Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration.ASIAN JOURNAL OF CHEMISTRY,23(4),1643-1647.
MLA	Jiang, Weixing,et al."Liquid Chromatography Tandem Mass Spectrometry Detection of Ablmosechus manihot and Its Major Metabolites in Rat Urine After Oral Administration".ASIAN JOURNAL OF CHEMISTRY 23.4(2011):1643-1647.