Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins

doi:10.1155/2015/278014

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	Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins
	Shi, Meina 1,3,4; Liu, Yingting 2; Feng, Lixing 4; Cui, Yingbo 1,3; Chen, Yajuan 1,3; Wang, Peng 1,3; Wu, Wenjuan 1,3; Chen, Chen 1,3; Liu, Xuan 4; Yang, Weimin 1,3
刊名	EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
	2015
ISSN号	1741-427X
DOI	10.1155/2015/278014
文献子类	Article
英文摘要	Scutellarin (SCU) is one of the main components of traditional Chinese medicine plant Erigeron breviscapus (Vant.) Hand.-Mazz. In this paper, we studied the protective effects of SCU on human cardiac microvascular endothelial cells (HCMECs) against hypoxia-reoxygenation (HR) injury and its possible target-related proteins. Results of MTT assay showed that pretreatment of SCU at doses of 1, 5, and 10.. M for 2 h could significantly inhibit the decrease in cell viability of HCMECs induced by HR injury. Subcellular fractions of cells treated with vehicle control, 1 mu M SCU, HR injury, or 1 mu M SCU + HR injury were separated by ultracentrifugation. The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). Proteins differentially expressed between control and SCU-treated group, control and HR group, or HR and SCU + HR group were identified using mass spectrometry (MS/MS). Possible interaction network of these target-related proteins was predicted using bioinformatic analysis. The influence of SCU on the expression levels of these proteins was confirmed using Western blotting assay. The results indicated that proteins such as p27BBP protein (EIF6), heat shock 60 kDa protein 1 (HSPD1), and chaperonin containing TCP1 subunit 6A isoform(CCT6A) might play important roles in the effects of SCU.
资助项目	National Natural Science Foundation of China[30960450] ; National Natural Science Foundation of China[81373964] ; National Natural Science Foundation of China[81173110] ; Yunnan Provincial Science and Technology Department[2011FA022] ; Yunnan Provincial Science and Technology Department[2014FA010] ; Yunnan Provincial Science and Technology Department[2014BC012] ; Shanghai Science & Technology Support Program[13431900401] ; National Science & Technology Major Project of China[2014ZX09301-306-03]
WOS关键词	MYOCARDIAL ISCHEMIA-REPERFUSION ; IN-VIVO ; MITOCHONDRIAL HSP60 ; CEREBRAL-ISCHEMIA ; BREVISCAPINE ; TRANSLATION ; EXPRESSION ; GENE ; RATS ; OVEREXPRESSION
WOS研究方向	Integrative & Complementary Medicine
语种	英语
出版者	HINDAWI PUBLISHING CORP
WOS记录号	WOS:000364049100001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276692]
专题	上海中药现代化研究中心
通讯作者	Liu, Xuan
作者单位	1.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Peoples R China; 2.Kunming Univ Sci & Technol, Kunhua Hosp, Peoples Hosp Yun Nan Prov 1, Dept Periodontol & Implant Dent, Kunming 650032, Peoples R China 3.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Shi, Meina,Liu, Yingting,Feng, Lixing,et al. Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2015.
APA	Shi, Meina.,Liu, Yingting.,Feng, Lixing.,Cui, Yingbo.,Chen, Yajuan.,...&Yang, Weimin.(2015).Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.
MLA	Shi, Meina,et al."Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2015).