LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription

doi:10.1365/s10337-007-0476-3

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海中药现代化研究中心

	LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription
	Zhang, Jin-Qiang 1,2; Huang, Hui-Lian 2; Chen, Guang-Tong 1,2; Lu, Zhi-Qiang 2; Tao, Si-Jia 2; Yang, Min 2; Sha, Na 2; Li, Xing-Nuo 1,2; Bi, Kai-Shun 1; Guo, De-An2
刊名	CHROMATOGRAPHIA
	2008-02
卷号	67 期号:3-4 页码:325-329
关键词	column liquid chromatography metabolism urinary and biliary excretion Luan-Pao-prescription
ISSN号	0009-5893
DOI	10.1365/s10337-007-0476-3
文献子类	Article
英文摘要	A simple and sensitive high-performance liquid chromatographic method with diode-array detection has been developed and validated for determination of six bioactive constituents, baicalin (BG), wogonoside (WG), baicalein (BA), wogonin (WO), oroxylin A (OA), and rhein (RHE), in rat urine and bile after oral administration of Luan-Pao-Prescription. The separation was performed on a C-18 column at 30 degrees C with a mobile phase gradient prepared from 0.5% aqueous glacial acetic acid and acetonitrile. Detection was at 320 nm for BG, WG, BA, WO, and OA, and 254 nm for RHE, for both biological fluids. Because of the properties of the compounds of interest in urine and bile, two methods of sample preparation were used-solid phase extraction for monitoring BG and WG and liquid-liquid extraction for BA, WO, OA, and RHE. The HPLC method was validated for linearity, LOD, LLOQ, precision, accuracy, and recovery from the biological fluids in accordance with ICH guidelines. The method was successfully used to investigate urinary and biliary excretion of the six constituents in rats.
WOS关键词	SOLID-PHASE EXTRACTION ; SCUTELLARIA-BAICALENSIS GEORGI ; CANCER CELL-LINES ; IN-VITRO ; LIQUID-CHROMATOGRAPHY ; BAICALIN ; WOGONIN ; RADIX ; METABOLITES ; PLASMA
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000252806200020
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272994]
专题	上海中药现代化研究中心
通讯作者	Guo, De-An
作者单位	1.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Zhang, Jin-Qiang,Huang, Hui-Lian,Chen, Guang-Tong,et al. LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription[J]. CHROMATOGRAPHIA,2008,67(3-4):325-329.
APA	Zhang, Jin-Qiang.,Huang, Hui-Lian.,Chen, Guang-Tong.,Lu, Zhi-Qiang.,Tao, Si-Jia.,...&Guo, De-An.(2008).LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription.CHROMATOGRAPHIA,67(3-4),325-329.
MLA	Zhang, Jin-Qiang,et al."LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription".CHROMATOGRAPHIA 67.3-4(2008):325-329.