Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres

doi:10.1016/j.apsb.2015.03.008

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	Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres
	Wang, Manli 1,2; Lu, Xiaolong 2,3; Yin, Xianzhen 2; Tong, Yajun 4; Peng, Weiwei 4; Wu, Li 2,5; Li, Haiyan2 ; Yang, Yan 5; Gu, Jingkai 5; Xiao, Tiqiao 4
刊名	ACTA PHARMACEUTICA SINICA B
	2015-05
卷号	5 期号:3 页码:270-276
关键词	Fourier-transform infrared spectromicroscopy Micerosphere Exenatide PLGA
ISSN号	2211-3835
DOI	10.1016/j.apsb.2015.03.008
文献子类	Article
英文摘要	The present study establishes a visualization method for the measurement of the distribution and localization of protein/peptide constituents within a single poly-lactide-co-glycolide (PLGA) microsphere using synchrotron radiation based Fourier-transform infrared speciiomlcroscopy (SR-FTIR). The representative infrared wavenumbers specific for protein/peptide (Exenatide) and excipient (PLGA) were identified and chemical maps at the single microsphere level were generated by measuring and plotting the intensity of these specific bands. For quantitative analysis of the distribution within microspheres, Matlab soft are was used to transform the map file into a 3D matrix and the matiix values specific for the drug and excipient were extracted. Comparison of the normalized SR- FM maps of PLGA and Exenatide indicated that PLGA was unit-on-lily distributed, while Exenatide was relatively non-uniformly distributed in the microspheres. In conclusion, SR-FTIR is a rapid, nondestructive and sensitive detection technology to provide the distribution of chemical constituents and functional groups in microparticles and microspheres. (C) 2015 Chinese Pharmaceutical Association and Institute of Materia 'Medico, Chinese Academy of 'Medical Sciences. Production and hosting by Elsevier B.V.
资助项目	National Natural Science Foundation of China[81273453] ; National Natural Science Foundation of China[81430087]
WOS关键词	FTIR SPECTROMICROSCOPY ; FLUORESCENCE MICROSCOPY ; RELEASE BEHAVIOR ; DRUG-DELIVERY ; MICROSPECTROSCOPY ; NANOPARTICLES ; PROTEIN ; MECHANISM ; CELLS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5448877
出版者	INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
WOS记录号	WOS:000360008900014
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276541]
专题	药物制剂研究中心分子影像中心（筹）
通讯作者	Chen, Min
作者单位	1.Anhui Univ Chinese Med, Sch Pharmaceut Sci, Hefei 230038, Peoples R China; 2.Chinese Acad Sci, Ctr Pharmaceut Preparat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Shanghai Inst Technol, Sch Mech Engn, Shanghai 201418, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China; 5.Jilin Univ, Coll Life Sci, Changchun 130012, Peoples R China
推荐引用方式 GB/T 7714	Wang, Manli,Lu, Xiaolong,Yin, Xianzhen,et al. Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres[J]. ACTA PHARMACEUTICA SINICA B,2015,5(3):270-276.
APA	Wang, Manli.,Lu, Xiaolong.,Yin, Xianzhen.,Tong, Yajun.,Peng, Weiwei.,...&Zhang, Jiwen.(2015).Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres.ACTA PHARMACEUTICA SINICA B,5(3),270-276.
MLA	Wang, Manli,et al."Synchrotron radiation-based Fourier-transform infrared spectromicroscopy for characterization of the protein/peptide distribution in single microspheres".ACTA PHARMACEUTICA SINICA B 5.3(2015):270-276.