Simultaneous high-throughput determination of interaction kinetics for drugs and cyclodextrins by high performance affinity chromatography with mass spectrometry detection

doi:10.1016/j.aca.2015.12.026

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	Simultaneous high-throughput determination of interaction kinetics for drugs and cyclodextrins by high performance affinity chromatography with mass spectrometry detection
	Wang, Caifen 2,3; Wang, Xiaobo 2; Xu, Xiaonan 2,3; Liu, Botao 1; Xu, Xu 1; Sun, Lixin 2; Li, Haiyan3 ; Zhang, Jiwen1,3
刊名	ANALYTICA CHIMICA ACTA
	2016-02-16
卷号	909 页码:75-83
关键词	Cyclodextrin supramolecular systems High performance affinity chromatography with mass spectrometry detection High-throughput quantifying Interactions kinetics
ISSN号	0003-2670
DOI	10.1016/j.aca.2015.12.026
文献子类	Article
英文摘要	The individual determination of the apparent dissociation rate constant (k(d,app)) using high performance affinity chromatography (HPAC) is a tedious process requiring numerous separate tests and massive data fitting, unable to provide the apparent association rate constant (k(a)) and equilibrium binding constant (K-a). In this study, a HPAC with mass spectrometry detection (HPAC-MS/MS) was employed to determine the drug-cyclodextrin (CD) interaction kinetics with low sample loading quantity (< 10 ng per injection for single compound) and high-throughput yield as twenty drugs determined in one injection. The kd, app measured by HPAC-MS/MS approach were 0.89 +/- 0.07, 4.34 +/- 0.01, 1.48 +/- 0.01 and 7.77 +/- 0.04 s(-1) for ketoprofen, trimethoprim, indapamide and acetaminophen, with k(d,app) for acetaminophen consistent with that from the HPAC method with UV detector in our previous studies. For twenty drugs with diverse structures and chemical properties, good correlationship was found between k(d,app) measured by single compound analysis method and high-throughput HPAC-MS/MS approach, with the correlation coefficient of 0.987 and the significance F less than 0.001. Comprehensive quantification of k(a,app), k(d,app) and K-a values was further performed based on the measurement of k(d,app) by peak profiling method and K-a by the peak fitting method. And the investigation of the drug-CD interaction kinetics under different conditions indicated that the column temperature and mobile phase composition significantly affected the determination of k(a,app), k(d,app) and K-a while also dependent on the acidity and basicity of drugs. In summary, the high-throughput HPAC-MS/MS approach has been demonstrated high efficiency in determination of the drug-CD primary interaction kinetic parameter, especially, k(d,app), being proven as a novel tool in screening the right CD for the solubilization of the right drug. (C) 2016 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81373358] ; National Natural Science Foundation of China[8157130864]
WOS关键词	HUMAN SERUM-ALBUMIN ; PROTEIN DISSOCIATION RATES ; PEAK DECAY ANALYSIS ; BINDING-KINETICS ; CHIRAL SOLUTES ; RATE CONSTANTS ; L-TRYPTOPHAN ; NANOPARTICLES ; DELIVERY ; COLUMNS
WOS研究方向	Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000369093600009
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276144]
专题	药物制剂研究中心
通讯作者	Sun, Lixin; Li, Haiyan; Zhang, Jiwen
作者单位	1.Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China 2.Shenyang Pharmaceut Univ, Sch Pharm, Dept Pharmaceut Anal, Shenyang 110016, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, 501 Haike Rd, Shanghai 201203, Peoples R China;
推荐引用方式 GB/T 7714	Wang, Caifen,Wang, Xiaobo,Xu, Xiaonan,et al. Simultaneous high-throughput determination of interaction kinetics for drugs and cyclodextrins by high performance affinity chromatography with mass spectrometry detection[J]. ANALYTICA CHIMICA ACTA,2016,909:75-83.
APA	Wang, Caifen.,Wang, Xiaobo.,Xu, Xiaonan.,Liu, Botao.,Xu, Xu.,...&Zhang, Jiwen.(2016).Simultaneous high-throughput determination of interaction kinetics for drugs and cyclodextrins by high performance affinity chromatography with mass spectrometry detection.ANALYTICA CHIMICA ACTA,909,75-83.
MLA	Wang, Caifen,et al."Simultaneous high-throughput determination of interaction kinetics for drugs and cyclodextrins by high performance affinity chromatography with mass spectrometry detection".ANALYTICA CHIMICA ACTA 909(2016):75-83.