Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract

doi:10.1002/bmc.4162

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	Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract
	Wang, Ludi 1; Yang, Wei 2; Wu, Siyang 2; Wang, Shuyao 2; Kang, Chen 2; Ma, Xiaoli 3; Li, Yingfei 2; Li, Chuan2,4
刊名	BIOMEDICAL CHROMATOGRAPHY
	2018-05
卷号	32 期号:5
关键词	daphnoretin isochamaejasmin neochamaejasmin A pharmacokineitc study Stellera chamaejasme L UPLC-MS MS
ISSN号	0269-3879
DOI	10.1002/bmc.4162
文献子类	Article
英文摘要	Isochamaejasmin, neochamaejasmin A and daphnoretin derived from Stellera chamaejasme L. are important because of their reported anticancer properties. In this study, a sensitive UPLC-MS/MS method for the determination of isochamaejasmin, neochamaejasmin A and daphnoretin in rat plasma was developed. The analyte and IS were separated on an Acquity UPLC HSS T3 column (100 x 2.1mm, 1.8m) using gradient elution with the mobile phase of aqueous solution (methanol-water, 1:99, v/v, containing 1mm formic acid) and organic solution (methanol-water, 99:1, v/v, containing 1mm formic acid) at a flow rate of 0.3mL/min. Multiple reaction monitoring mode with negative electrospray ionization interface was carried out to detect the components. The method was validated in terms of specificity, linearity, accuracy, precision, stability, etc. Excellent linear behavior was observed over the certain concentration ranges with the correlation coefficient values >0.99. Intra- and inter-day precisions (RSD) were <6.7% and accuracy (RE) ranged from -7.0 to 12.0%. The validated method was successfully applied to investigate the pharmacokinetics of three chemical ingredients after oral administration of S. chamaejasme L. extract to rats.
资助项目	National Science & Technology Major Project of China 'Key New Drug Creation and Manufacturing Program[2015ZX09501004-003-005] ; Building Project of Excellent Science and Technology Innovation Team of China Academy of Chinese Medical Sciences[YS1308]
WOS关键词	CELL-CYCLE ARREST ; HELA-CELLS ; DAPHNORETIN ; APOPTOSIS ; CHROMATOGRAPHY ; PATHWAY
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	WILEY
WOS记录号	WOS:000430464400002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279781]
专题	上海药物代谢研究中心科研与新药推进处
通讯作者	Ma, Xiaoli; Li, Yingfei
作者单位	1.Hebei Univ, Coll Chem & Environm Sci, Baoding, Hebei, Peoples R China; 2.China Acad Chinese Med Sci, Inst Chinese Mat Med, Ctr DMPK Res Herbal Med, Beijing 100700, Peoples R China; 3.Hebei Univ, Coll Tradit Chinese Med, Baoding 071000, Hebei, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Lab DMPK Res Herbal Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ludi,Yang, Wei,Wu, Siyang,et al. Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract[J]. BIOMEDICAL CHROMATOGRAPHY,2018,32(5).
APA	Wang, Ludi.,Yang, Wei.,Wu, Siyang.,Wang, Shuyao.,Kang, Chen.,...&Li, Chuan.(2018).Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract.BIOMEDICAL CHROMATOGRAPHY,32(5).
MLA	Wang, Ludi,et al."Simultaneous determination of isochamaejasmin, neochamaejasmin A and aphnoretinin rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Stellera chamaejasme L. extract".BIOMEDICAL CHROMATOGRAPHY 32.5(2018).