Structure and chain conformation of a (1 -> 6)-alpha-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative

doi:10.1016/j.carbpol.2008.04.034

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	Structure and chain conformation of a (1 -> 6)-alpha-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative
	Cui, Hengxiang 1,2; Liu, Qin 1; Tao, Yongzhen 3; Zhang, Hongfeng 2; Zhang, Lina 3; Ding, Kan1
刊名	CARBOHYDRATE POLYMERS
	2008-11-21
卷号	74 期号:4 页码:771-778
关键词	Pueraria lobata (1 -> 6)-alpha-D-glucan chain conformation antioxidant effect sulfated polysaccharide
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2008.04.034
文献子类	Article
英文摘要	A water soluble glucan, PLB-2C, was isolated from the water extract of the root of Pueraria lobota (Willd) Ohwi using anion-exchange and gel permeation chromatography. Its structure was investigated by gas chromatography (GC) gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra, and nuclear magnetic resonance (NMR) spectroscopy of heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) techniques. The results indicated that PLB-2C was a linear glucan composed of (1 -> 6)-alpha-D-Glcp. Chain conformation study showed that the polysaccharide took random coil compact conformation. In vitro cell viability assay by MTT method, its sulfated derivative PLB-2CS which was substituted at 2-0, 3-0, 4-0 positions, at 0.1, 1, and 5 mg/ml, could attenuate PC12 cell damage significantly caused by hydrogen peroxide. (C) 2008 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China (NSFC)[30670470] ; High Tech Research and Development (863) Program[2006AA02Z102] ; Shanghai Institute of Materia Medica[00000000] ; Chinese Academy of Sciences[07G604F036]
WOS关键词	MAGNETIC-RESONANCE SPECTROSCOPY ; MAJOR ISOFLAVONOID CONTENTS ; HEPARAN-SULFATE ; IN-VITRO ; POLYSACCHARIDES ; GLUCAN ; PROTEOGLYCANS ; METHYLATION ; ACTIVATION ; MIRIFICA
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000259765300003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279428]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 2.E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China; 3.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Cui, Hengxiang,Liu, Qin,Tao, Yongzhen,et al. Structure and chain conformation of a (1 -> 6)-alpha-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative[J]. CARBOHYDRATE POLYMERS,2008,74(4):771-778.
APA	Cui, Hengxiang,Liu, Qin,Tao, Yongzhen,Zhang, Hongfeng,Zhang, Lina,&Ding, Kan.(2008).Structure and chain conformation of a (1 -> 6)-alpha-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative.CARBOHYDRATE POLYMERS,74(4),771-778.
MLA	Cui, Hengxiang,et al."Structure and chain conformation of a (1 -> 6)-alpha-D-glucan from the root of Pueraria lobata (Willd.) Ohwi and the antioxidant activity of its sulfated derivative".CARBOHYDRATE POLYMERS 74.4(2008):771-778.