Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa

doi:10.1128/JB.01538-14

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第三研究室

	Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa
	Deng, Xin 2,3; Liang, Haihua 2,3; Ulanovskaya, Olesya A.1,4; Ji, Quanjiang 2,3; Zhou, Tianhong 2,3; Sun, Fei 2,3; Lu, Zhike 2,3; Hutchison, Alan L.2,3; Lan, Lefu5 ; Wu, Min 6
刊名	JOURNAL OF BACTERIOLOGY
	2014-07
卷号	196 期号:14 页码:2499-2513
ISSN号	0021-9193
DOI	10.1128/JB.01538-14
文献子类	Article
英文摘要	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from human pathogens Staphylococcus aureus and Pseudomonas aeruginosa can be readily inhibited by reactive oxygen species (ROS)-mediated direct oxidation of their catalytic active cysteines. Because of the rapid degradation of H2O2 by bacterial catalase, only steady-state but not one-dose treatment with H2O2 rapidly induces glycolysis and the pentose phosphate pathway (PPP). We conducted transcriptome sequencing (RNA-seq) analyses to globally profile the bacterial transcriptomes in response to a steady level of H2O2, which revealed profound transcriptional changes, including the induced expression of glycolytic genes in both bacteria. Our results revealed that the inactivation of GAPDH by H2O2 induces metabolic levels of glycolysis and the PPP; the elevated levels of fructose 1,6-biphosphate (FBP) and 2-keto-3-deoxy-6-phosphogluconate (KDPG) lead to dissociation of their corresponding glycolytic repressors (GapR and HexR, respectively) from their cognate promoters, thus resulting in derepression of the glycolytic genes to overcome H2O2-stalled glycolysis in S. aureus and P. aeruginosa, respectively. Both GapR and HexR may directly sense oxidative stresses, such as menadione.
资助项目	National Institutes of Health[AI074658] ; National Institutes of Health[P50GM081892] ; Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Disease Award[00000000]
WOS关键词	OXIDATION-SENSING MECHANISM ; PENTOSE-PHOSPHATE PATHWAY ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; TRANSCRIPTIONAL REGULATION ; ANTIOXIDANT RESPONSE ; BACILLUS-SUBTILIS ; RNA-SEQ ; STRESS ; REGULATOR ; REDUCTASE
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000338765600001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277006]
专题	药理学第三研究室
通讯作者	He, Chuan
作者单位	1.Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA; 2.Univ Chicago, Dept Chem, Chicago, IL 60637 USA; 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA; 4.Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China; 6.Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA
推荐引用方式 GB/T 7714	Deng, Xin,Liang, Haihua,Ulanovskaya, Olesya A.,et al. Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa[J]. JOURNAL OF BACTERIOLOGY,2014,196(14):2499-2513.
APA	Deng, Xin.,Liang, Haihua.,Ulanovskaya, Olesya A..,Ji, Quanjiang.,Zhou, Tianhong.,...&He, Chuan.(2014).Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa.JOURNAL OF BACTERIOLOGY,196(14),2499-2513.
MLA	Deng, Xin,et al."Steady-State Hydrogen Peroxide Induces Glycolysis in Staphylococcus aureus and Pseudomonas aeruginosa".JOURNAL OF BACTERIOLOGY 196.14(2014):2499-2513.