A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4

doi:10.1016/j.carbpol.2016.02.068

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	A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4
	Wang, Wucheng; Chen, Huanjun; Zhang, Lei; Qin, Yi; Cong, Qifei; Wang, Peipei; Ding, Kan
刊名	CARBOHYDRATE POLYMERS
	2016-06-25
卷号	144 页码:305-314
关键词	Nemacystus decipiens Fucoidan Structure Anti-angiogenic activity Bone morphogenetic protein 4
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2016.02.068
文献子类	Article
英文摘要	A sulfated and acetylated fucoidan, named NDH01, was extracted from seaweed Nemacystus decipiens. NDH01 was composed of mannose, glucuronic acid, fucose, sulfate group and acetyl group in the molar ratio of 3.0: 14.4: 82.6: 34.3: 13.9. The backbone of NDH01 was fucose-free core, composed of alpha-D-1,2-Manp and beta-D-1,4-GlcpA disaccharide repeat unit. The branches were attached at the C3, C4 and C6 of alpha-D-1,2-Manp. The sidechain was composed of alpha-L-1,3,4-Fucp, alpha-L-1,4-Fucp, alpha-L-1,3-Fucp and alpha-L-1,4-GlcpA. The sulfate group was linked to C4 of alpha-L-1,3,4-Fucp, whereas, acetyl group was branched on C2 of alpha-L-1,2,3-Fucp. NDH01 could disrupt tube formation and inhibit the migration as well as cell growth of human microvascular endothelial cells. Besides, phosphorylation of Smad/1/5/8, Erk and FAK was significantly inhibited by NDH01. Further studies uncovered that NDH01 blocked Smad1/5/8 signaling via interacting with bone morphogenetic protein 4 and downregulating bone morphogenetic protein 4 expression. The results suggested that NDH01 might be an angiogenesis inhibitor through targeting bone morphogenetic protein 4. (C) 2016 Elsevier Ltd. All rights reserved.
资助项目	New Drug Creation and Manufacturing Program[2012ZX09301001-003] ; key program of National Natural Science Foundation of China[31230022] ; National Natural Science Foundation of China[31400690]
WOS关键词	BROWN SEAWEED ; SULFATED POLYSACCHARIDE ; BIOLOGICAL-ACTIVITIES ; TUMOR ANGIOGENESIS ; EXPRESSION ; PATHWAY ; CANCER ; CELLS ; CORE ; BMP
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000373830700037
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275986]
专题	药理学第三研究室
通讯作者	Ding, Kan
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wang, Wucheng,Chen, Huanjun,Zhang, Lei,et al. A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4[J]. CARBOHYDRATE POLYMERS,2016,144:305-314.
APA	Wang, Wucheng.,Chen, Huanjun.,Zhang, Lei.,Qin, Yi.,Cong, Qifei.,...&Ding, Kan.(2016).A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4.CARBOHYDRATE POLYMERS,144,305-314.
MLA	Wang, Wucheng,et al."A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4".CARBOHYDRATE POLYMERS 144(2016):305-314.