Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli

doi:10.1016/j.bbapap.2010.07.020

	Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli
	Peng, Yu 2; Liu, Jiafu 2; Liu, Qiang 3; Yao, Yihe 2; Guo, Chenyun 2; Zhang, Yonglian 3; Lin, Donghai 1,2
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
	2010-11
卷号	1804 期号:11 页码:2102-2110
关键词	Lipocalin 12 (Lcn12) Epididymis beta-Barrel Cysteine Disulfide bond Retinoic acid
ISSN号	1570-9639
DOI	10.1016/j.bbapap.2010.07.020
文献子类	Article
英文摘要	Lipocalin 12 (Lcn12) is a recently identified epididymis-specific protein that might play a significant physiological role in male reproduction. However, the detailed structure and function of Lcn12 remain to be determined. In the present work, we cloned, expressed, and purified the rat Lcn12 (rLcn12) protein in Escherichia coli, introduced the Cys176Ala substitution to eliminate the aggregation problem associated with the wild-type protein. Homology modeling results demonstrated that rLcn12 adopted an eight-stranded, antiparallel beta-barrel conformation containing a conserved disulfide bond between Cys98 and Cys203, which was in accordance with the physicochemical properties elucidated by a combination of mass, circular dichroism, and nuclear magnetic resonance spectrometry. The purified rLcn12 protein exhibited a high binding affinity for all-trans retinoic acid in fluorescence titration experiments, implying that rLcn12 could be involved in retinoic acid transport in the epididymis. (C) 2010 Elsevier B.V. All rights reserved.
资助项目	Natural Science Foundation of China[30730026] ; Chinese Academy of Sciences (CAS)[KSCX1-YW-R-54] ; Chinese Academy of Sciences (CAS)[KSCX2-YW-R-104] ; National Science & Technology Major Project"Key New Drug Creation and Manufacturing Program," China[2009ZX09301-001]
WOS关键词	ACID-BINDING PROTEIN ; CONSERVED DISULFIDE BOND ; PROSTAGLANDIN-D SYNTHASE ; LIGAND-BINDING ; FAMILY ; SEQUENCE ; NGAL ; STABILITY ; EVOLUTION ; RETINOIDS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000282720700002
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278728]
专题	分析化学研究室
通讯作者	Lin, Donghai
作者单位	1.Xiamen Univ, Coll Chem & Chem Engn, Dept Biol Chem, Key Lab Chem Biol Fujian Prov, Xiamen 361005, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Biomol NMR, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China;
推荐引用方式 GB/T 7714	Peng, Yu,Liu, Jiafu,Liu, Qiang,et al. Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,2010,1804(11):2102-2110.
APA	Peng, Yu.,Liu, Jiafu.,Liu, Qiang.,Yao, Yihe.,Guo, Chenyun.,...&Lin, Donghai.(2010).Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS,1804(11),2102-2110.
MLA	Peng, Yu,et al."Conformational and biochemical characterization of a rat epididymis-specific lipocalin 12 expressed in Escherichia coli".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1804.11(2010):2102-2110.