Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin

doi:10.3724/SP.J.1096.2011.01223

	Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin
	Wu Li-Min 2; Zhang Mei-Ling 2; Lou Yi-Yi 2; Li Yang2 ; Zhao Liang-Cai 1; Huang Ke-Xin 2; Gao Hong-Chang 2
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2011-08
卷号	39 期号:8 页码:1223-1227
关键词	Nuclear magnetic resonance(NMR) Berberine Bovine serum albumin Relaxation time Self-diffusion coefficient
ISSN号	0253-3820
DOI	10.3724/SP.J.1096.2011.01223
文献子类	Article
英文摘要	(1)H nuclear magnetic resonance chemical shift, relaxation time and diffusion coefficient measurements were carried out to study the interaction on the affinity binding of Berberine, an antibacterial drug, to bovine serum albumin (BSA). The results showed that the protons of aromatic ring of Berberine were more favorable to form Berberine-BSA complex than those of aliphatic groups. BSA combination resulted in significantly decrease in the relaxation time of Berberine protons, which suggests that the motion of these protons were limited in aqueous solutions. The saturation concentration ratio of Berberine to BSA is 60:1 according to the measurements of chemical shift and relaxation time. The apparent dissociation constant was 0.44 mmol/L and the binding sites of Berberine molecules on BSA was 22 fitted from the self-diffusion measurements. This study is of great importance for the safe and rational use of Berberine in clinic, and development of improved Berberine analogs.
WOS关键词	DIFFUSION MEASUREMENTS ; NMR DIFFUSION ; RELAXATION ; H-1-NMR ; BINDING
WOS研究方向	Chemistry
语种	中文
CSCD记录号	CSCD:4270692
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000296599800018
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278450]
专题	分析化学研究室
通讯作者	Gao Hong-Chang
作者单位	1.Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Wenzhou Med Coll, Sch Pharm, Wenzhou 325035, Peoples R China;
推荐引用方式 GB/T 7714	Wu Li-Min,Zhang Mei-Ling,Lou Yi-Yi,et al. Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(8):1223-1227.
APA	Wu Li-Min.,Zhang Mei-Ling.,Lou Yi-Yi.,Li Yang.,Zhao Liang-Cai.,...&Gao Hong-Chang.(2011).Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(8),1223-1227.
MLA	Wu Li-Min,et al."Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.8(2011):1223-1227.