Identification of a Novel Function of Adipocyte Plasma Membrane Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue

doi:10.1021/acs.jproteome.5b01030

	Identification of a Novel Function of Adipocyte Plasma Membrane Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue
	Ma, Yuhang 1; Gao, Jing 2,3; Yin, Jiajing 1; Gu, Liping 1; Liu, Xing 2,3; Chen, Su 1; Huang, Qianfang 1; Lu, Huifang 4; Yang, Yuemin 4; Zhou, Hu2,3
刊名	JOURNAL OF PROTEOME RESEARCH
	2016-02
卷号	15 期号:2 页码:628-637
关键词	gestational diabetes mellitus label-free proteomic analysis insulin resistance inflammation
ISSN号	1535-3893
DOI	10.1021/acs.jproteome.5b01030
文献子类	Article
英文摘要	Gestational diabetes mellitus (GDM) is considered as an early stage of type 2 diabetes mellitus. In this study, we compared demographic and clinical data between six GDM subjects and six normal glucose tolerance (NGT; healthy controls) subjects and found that homeostasis model of assessment for insulin resistance index (HOMA-IR) increased in GDM. Many previous studies demonstrated that omental adipose tissue dysfunction could induce insulin resistance. Thus, to investigate the cause of insulin resistance in GDM, we used label-free proteomics to identify differentially expressed proteins in omental adipose tissues from GDM and NGT subjects (data are available via ProteomeXchange with identifier PXD003095). A total of 3528 proteins were identified, including 66 significantly changed proteins. Adipocyte plasma membrane-associated protein (APMAP, a.k.a. C20orf3), one of the differentially expressed proteins, was down-regulated in GDM omental adipose tissues. Furthermore, mature 3T3-L1 adipocytes were used to simulate omental adipocytes. The inhibition of APMAP expression by RNAi impaired insulin signaling and activated NF kappa B signaling in these adipocytes. Our study revealed that the down-regulation of APMAP in omental adipose tissue may play an important role in insulin resistance in the pathophysiology of GDM.
资助项目	National Science Foundation of China[8130063] ; National Science Foundation of China[21375138] ; Open Research Project of the Shanghai Key Laboratory of Diabetes Mellitus[SHKLD-KF-1301] ; Shanghai Municipal Education Commission - Gaofeng Clinical Medicine Grant[20152231] ; Shanghai Pujiang Program[15PJD032] ; Ministry of Science and Technology of China, "one hundred talent program" of Chinese Academy of Sciences[2013ZX09507001] ; Innovation Project of Instrument and Equipment Function Development[00000000] ; Bureau of Goods, Chinese Academy of Sciences[YZ201542]
WOS关键词	INSULIN-RESISTANCE ; KAPPA-B ; INTERNATIONAL ASSOCIATION ; METABOLIC DISEASE ; INFLAMMATION ; OBESITY ; GLUCOSE ; PHOSPHORYLATION ; ELECTROPHORESIS ; DIFFERENTIATION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000369771700026
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/276164]
专题	分析化学研究室
通讯作者	Zhou, Hu; Wang, Yufan; Peng, Yongde
作者单位	1.Shanghai Jiao Tong Univ, Sch Med, Dept Endocrinol & Metab, Shanghai Gen Hosp, Shanghai 200080, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Dept Obstet & Gynecol, Shanghai Gen Hosp, Shanghai 200080, Peoples R China;
推荐引用方式 GB/T 7714	Ma, Yuhang,Gao, Jing,Yin, Jiajing,et al. Identification of a Novel Function of Adipocyte Plasma Membrane Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue[J]. JOURNAL OF PROTEOME RESEARCH,2016,15(2):628-637.
APA	Ma, Yuhang.,Gao, Jing.,Yin, Jiajing.,Gu, Liping.,Liu, Xing.,...&Peng, Yongde.(2016).Identification of a Novel Function of Adipocyte Plasma Membrane Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue.JOURNAL OF PROTEOME RESEARCH,15(2),628-637.
MLA	Ma, Yuhang,et al."Identification of a Novel Function of Adipocyte Plasma Membrane Associated Protein (APMAP) in Gestational Diabetes Mellitus by Proteomic Analysis of Omental Adipose Tissue".JOURNAL OF PROTEOME RESEARCH 15.2(2016):628-637.