A redox mechanism underlying nucleolar stress sensing by nucleophosmin

doi:10.1038/ncomms13599

	A redox mechanism underlying nucleolar stress sensing by nucleophosmin
	Yang, Kai 2; Wang, Ming 2; Zhao, Yuzheng 3; Sun, Xuxu 2; Yang, Yi 3; Li, Xie 3; Zhou, Aiwu 4; Chu, Huilin 2; Zhou, Hu5 ; Xu, Jianrong 6
刊名	NATURE COMMUNICATIONS
	2016-11-25
卷号	7
ISSN号	2041-1723
DOI	10.1038/ncomms13599
文献子类	Article
英文摘要	The nucleolus has been recently described as a stress sensor. The nucleoplasmic translocation of nucleolar protein nucleophosmin (NPM1) is a hallmark of nucleolar stress; however, the causes of this translocation and its connection to p53 activation are unclear. Using single live-cell imaging and the redox biosensors, we demonstrate that nucleolar oxidation is a general response to various cellular stresses. During nucleolar oxidation, NPM1 undergoes S-glutathionylation on cysteine 275, which triggers the dissociation of NPM1 from nucleolar nucleic acids. The C275S mutant NPM1, unable to be glutathionylated, remains in the nucleolus under nucleolar stress. Compared with wild-type NPM1 that can disrupt the p53-HDM2 interaction, the C275S mutant greatly compromises the activation of p53, highlighting that nucleoplasmic translocation of NPM1 is a prerequisite for stress-induced activation of p53. This study elucidates a redox mechanism for the nucleolar stress sensing and may help the development of therapeutic strategies.
资助项目	National Natural Science Foundation of China[31230037] ; National Natural Science Foundation of China[31471263] ; Ministry of Science and Technology of China[2013CB910900] ; Shanghai Municipal Science and Technology Commission[11JC1406900] ; Shanghai Municipal Science and Technology Commission[11DZ2260200] ; Shanghai Municipal Science and Technology Commission[16ZR1418400] ; Shanghai Municipal Science and Technology Commission[15YF1402600] ; Lift Engineering for Young Talent of China Association for Science and Technology[00000000]
WOS关键词	DNA-DAMAGE ; CELL-PROLIFERATION ; RIBOSOMAL-RNA ; PROTEIN B23 ; S-GLUTATHIONYLATION ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; CANCER-CELLS ; HELA-CELLS ; P53
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000388641200003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275798]
专题	分析化学研究室
通讯作者	Yang, Yi; Yi, Jing
作者单位	1.Univ Sci & Technol China, Sch Life Sci, 96 Jinzhai Rd, Hefei 230022, Anhui, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med,Minist Educ, Dept Biochem & Mol Cell Biol,Key Lab Cell Differe, Shanghai Key Lab Tumor Microenvironm & Inflammat, 280 South Chongqing Rd, Shanghai 200025, Peoples R China; 3.East China Univ Sci & Technol, Sch Pharm, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Dept Pathophysiol, 280 South Chongqing Rd, Shanghai 200025, Peoples R China; 5.Shanghai Inst Mat Med, 555 Zu Chong Zhi Rd,Zhang Jiang Hi Tech Pk, Shanghai 201203, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Dept Pharmacol, 280 South Chongqing Rd, Shanghai 200025, Peoples R China;
推荐引用方式 GB/T 7714	Yang, Kai,Wang, Ming,Zhao, Yuzheng,et al. A redox mechanism underlying nucleolar stress sensing by nucleophosmin[J]. NATURE COMMUNICATIONS,2016,7.
APA	Yang, Kai.,Wang, Ming.,Zhao, Yuzheng.,Sun, Xuxu.,Yang, Yi.,...&Yi, Jing.(2016).A redox mechanism underlying nucleolar stress sensing by nucleophosmin.NATURE COMMUNICATIONS,7.
MLA	Yang, Kai,et al."A redox mechanism underlying nucleolar stress sensing by nucleophosmin".NATURE COMMUNICATIONS 7(2016).