Roles of p53, c-Myc, Bcl-2, Bax and caspases in glutamate-induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor

doi:10.1016/S0169-328X(99)00186-2

	Roles of p53, c-Myc, Bcl-2, Bax and caspases in glutamate-induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor
	Liu, X; Zhu, XZ
刊名	MOLECULAR BRAIN RESEARCH
	1999-08-25
卷号	71 期号:2 页码:210-216
关键词	glutamate neuron apoptosis Bcl-2 caspase basic fibroblast growth factor
ISSN号	0169-328X
DOI	10.1016/S0169-328X(99)00186-2
文献子类	Article
英文摘要	By using flow-cytometric analysis, we examined the involvement of p53, c-Myc, Bcl-2 and Bax in the glutamate-induced cell death in cultured cortical neurons. The activities of caspase-1-like and caspase-3-like proteases were also measured after the glutamate treatment. The apoptosis rate of the cells increased after 12 h and 24 h treatment with, glutamate. The temporal profile of p53, c-Myc, Bcl-2, Bax expression and caspases activation after glutamate treatment suggest that Bcl-2, c-Myc and caspase-3 play important roles in the excitotoxic neuronal cell death. The down-regulation of Bcl-2 may be an important early stage event, which may cause the activation of caspase-3, c-Myc is also involved in the process of apoptosis though its precise role remains elusive. bFGF exhibited the capability to antagonize the neuronal apoptosis caused by glutamate. The antiapoptotic potential of bFGF may result from its attenuating effect on the down-regulation of Bcl-2 induced by glutamate and, subsequently, blockade of apoptosis cascade. This may provide a possible explanation for its neuroprotective effect against ischemic cell death. (C) 1999 Elsevier Science B.V. All rights reserved.
WOS关键词	CEREBELLAR GRANULE CELLS ; FLOW-CYTOMETRIC ANALYSIS ; CEREBRAL-ISCHEMIA ; DEATH ; EXPRESSION ; FAMILY ; GENE ; MITOCHONDRIA ; ACTIVATION ; DISORDERS
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000082766700008
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274707]
专题	分析化学研究室药理学第二研究室药物安全性评价中心
通讯作者	Zhu, XZ
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Liu, X,Zhu, XZ. Roles of p53, c-Myc, Bcl-2, Bax and caspases in glutamate-induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor[J]. MOLECULAR BRAIN RESEARCH,1999,71(2):210-216.
APA	Liu, X,&Zhu, XZ.(1999).Roles of p53, c-Myc, Bcl-2, Bax and caspases in glutamate-induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor.MOLECULAR BRAIN RESEARCH,71(2),210-216.
MLA	Liu, X,et al."Roles of p53, c-Myc, Bcl-2, Bax and caspases in glutamate-induced neuronal apoptosis and the possible neuroprotective mechanism of basic fibroblast growth factor".MOLECULAR BRAIN RESEARCH 71.2(1999):210-216.