One-step affinity purification of human mu-opioid receptor overexpressed in baculovirus system

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	One-step affinity purification of human mu-opioid receptor overexpressed in baculovirus system
	Chen, LW; Feng, YP; Zhou, DH; Xu, XJ; Chen, J; Wei, Q; Chi, ZQ
刊名	PROTEIN AND PEPTIDE LETTERS
	2001-08
卷号	8 期号:4 页码:265-272
关键词	mu opioid receptors Baculoviridae Sf9 insect cells diprenorphine radioligand assay protein purification Ni-nitrilotriacetic acid agarose
ISSN号	0929-8665
文献子类	Article
英文摘要	Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.
WOS关键词	INFECTED INSECT CELLS ; ADRENERGIC-RECEPTOR
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	BENTHAM SCIENCE PUBL LTD
WOS记录号	WOS:000170302800004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274467]
专题	药理学第二研究室院士及顾问专家药物安全性评价中心
作者单位	Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Chen, LW,Feng, YP,Zhou, DH,et al. One-step affinity purification of human mu-opioid receptor overexpressed in baculovirus system[J]. PROTEIN AND PEPTIDE LETTERS,2001,8(4):265-272.
APA	Chen, LW.,Feng, YP.,Zhou, DH.,Xu, XJ.,Chen, J.,...&Chi, ZQ.(2001).One-step affinity purification of human mu-opioid receptor overexpressed in baculovirus system.PROTEIN AND PEPTIDE LETTERS,8(4),265-272.
MLA	Chen, LW,et al."One-step affinity purification of human mu-opioid receptor overexpressed in baculovirus system".PROTEIN AND PEPTIDE LETTERS 8.4(2001):265-272.