Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis

doi:10.1055/s-2002-23133

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 天然药物化学研究室

	Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis
	Bao, XF; Wang, Z; Fang, JN; Li, XY
刊名	PLANTA MEDICA
	2002-03
卷号	68 期号:3 页码:237-243
关键词	Cuscuta chinensis Convolvulaceae acidic polysaccharide lymphocyte proliferation antibody production free radical PC12 cell
ISSN号	0032-0943
DOI	10.1055/s-2002-23133
文献子类	Article
英文摘要	Three crude polysaccharide fractions, named CS-A, CS-B and CS-C, were prepared from the seeds of Cuscuta chinensis by hot water extraction and diluted alkali extraction subsequently, then EtOH precipitation, and tested for lymphocyte proliferation activity. CS-A showed a stimulating effect on concanavalin A or lipopolysaccharide induced mitogenic activity of lymphocytes. An acidic polysaccharide (CS-A-3beta) was purified from CS-A by anion exchange and gel filtration chromatography. The polysaccharide showed potent stimulating effects on lymphocyte proliferation and antibody production, but did not significantly influence the serum IgG level. Its structural features were elucidated by methylation analysis, partial acid hydrolysis, 1 D and 2D NMR and ESI-mass spectroscopy. The data obtained indicated that CS-A-3beta had a backbone consisting of alpha-D-1,4-linked GalpA residues and beta-L-1,2-linked Rhap residues with branches at C-4 of Rhap residues and C-3 of GalpA residues, composed of arabinogalactan and glucobiose. This structure is typical for a pectic polysaccharide of the rhamnogalacturonan type. In addition, the effect of CS-A, CS-B, CS-C and CS-A-3beta on hydrogen peroxide induced cell lesion in rat pheochromocytoma line PC 12 was investigated. The results indicated that, besides its immunostimulating activity, CS-A-3beta had a protective effect against free radical-induced cell toxicity.
WOS关键词	ROOTS ; GLC
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种	英语
出版者	GEORG THIEME VERLAG KG
WOS记录号	WOS:000174883700010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274384]
专题	天然药物化学研究室药理学第三研究室
通讯作者	Fang, JN
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Bao, XF,Wang, Z,Fang, JN,et al. Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis[J]. PLANTA MEDICA,2002,68(3):237-243.
APA	Bao, XF,Wang, Z,Fang, JN,&Li, XY.(2002).Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis.PLANTA MEDICA,68(3),237-243.
MLA	Bao, XF,et al."Structural features of an immunostimulating and antioxidant acidic polysaccharide from the seeds of Cuscuta chinensis".PLANTA MEDICA 68.3(2002):237-243.