Serine racemase expression in mouse cerebral cortex after permanent focal cerebral ischemia

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	Serine racemase expression in mouse cerebral cortex after permanent focal cerebral ischemia
	Wang, LZ; Zhu, XZ
刊名	ACTA PHARMACOLOGICA SINICA
	2004-04
卷号	25 期号:4 页码:436-441
关键词	D-serine L-serine serine racemase inbred C57BL mice brain ischemia
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To study the alterations of the expressions of serine racemase in C57BL/6 mouse brain after permanent focal cerebral ischemia. METHODS: The mRNA level and the protein level of serine racemase were assayed by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot, respectively. The amount of D-serine and L-serine were measured by HPLC. RESULTS: High levels of serine racemase were constitutively expressed in the normal cortex of mouse. At early stage after middle cerebral artery occlusion (MCAO), no significant change in expression of serine racemase was observed in temporoparietal cortex in ipsilateral hemisphere. However, delayed transient decreases of serine racemase in both mRNA and protein levels were detected from d 6 to d 10 after ischemia. Correspondingly, D-serine concentration also declined in the ipsilateral cortex during, this period when compared with the D-serine level in the contralateral cortex. CONCLUSION: Delayed decreases in serine racemase expression and D-serine level occurred in the temporoparietal cortex at the late stage after focal cerebral ischemia.
WOS关键词	GLYCINE SITE ; NMDA RECEPTOR ; RAT-BRAIN ; EXTRACELLULAR CONCENTRATION ; GLUTAMATE ; NEUROMODULATOR ; DAMAGE
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:1842194
出版者	SHANGHAI INST MATERIA MEDICA
WOS记录号	WOS:000220750000006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274094]
专题	药理学第二研究室
通讯作者	Zhu, XZ
作者单位	Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Wang, LZ,Zhu, XZ. Serine racemase expression in mouse cerebral cortex after permanent focal cerebral ischemia[J]. ACTA PHARMACOLOGICA SINICA,2004,25(4):436-441.
APA	Wang, LZ,&Zhu, XZ.(2004).Serine racemase expression in mouse cerebral cortex after permanent focal cerebral ischemia.ACTA PHARMACOLOGICA SINICA,25(4),436-441.
MLA	Wang, LZ,et al."Serine racemase expression in mouse cerebral cortex after permanent focal cerebral ischemia".ACTA PHARMACOLOGICA SINICA 25.4(2004):436-441.