Low molecular weight xanthan gum for treating osteoarthritis

doi:10.1016/j.carbpol.2017.01.101

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	Low molecular weight xanthan gum for treating osteoarthritis
	Han, Guanying 2,3,4,5; Chen, Qixin 2,3; Liu, Fei 2,3; Cui, Zan 5; Shao, Huarong 2,3; Liu, Fuyan 2,3; Ma, Aibin 2,3; Liao, Joshua 1; Guo, Bin5 ; Guo, Yuewei4
刊名	CARBOHYDRATE POLYMERS
	2017-05-15
卷号	164 页码:386-395
关键词	Xanthan gum Low molecular weight Sodium hyaluronate Osteoarthritis
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2017.01.101
文献子类	Article
英文摘要	Osteoarthritis (OA) is one of the most common chronic diseases and characterized by degradation of articular cartilage. We have previously reported xanthan gum (XG) injection preparation with high molecular weights (Mw) in ranging from 3 x 10(6) Da to 5 x 10(6) Da (HM-XG) could enhance the viscosity of synovial fluid, protect joint cartilage in rabbit, and the therapeutical effect has no significance difference with an existing clinical medication (sodium hyaluronate, SH) at the same injection frequency (once weekly for 5 weeks). Herein, we prepared a XG injection preparation with a low Mw (LM-XG) in ranging from 1 x 10(6) Da to 1.5 x 10(6) Da, and evaluated the therapeutical effect for OA therapy at once every 2 weeks for 5 weeks with an SH at once weekly for 5 weeks as reference. The model of OA was induced using anterior cruciate ligament transection (ACLT) in a rabbit in vivo and also using sodium nitroprusside (SNP) in cell culture in vitro. The results showed that LW-XG could also protect cartilage from damage, decrease the concentration of nitric oxide (NO) in synovial fluid and reverse the amplification of the knee joint width similar to HM-XG as our previously reported. At the cellular level, LW-XG promotes proliferation while decreases apoptosis of chondrocytes. Mechanistically at the molecular level, these effects are elicited via down-regulation of the protein levels of caspase-3 and bax and up-regulation of the protein levels of bcl-2 in cartilage in both in vivo and in vitro. These results showed that LW-XG maybe become an excellent candidate long-acting drug for treating OA. (C) 2017 Published by Elsevier Ltd.
资助项目	National Major Scientific and Technological Special Project for "Significant New Drugs Development" during the Twelfth Five-year Plan Period[2012ZX093016-002] ; National Natural Science Foundation of China[81302681] ; Development Plan of the Outstanding Young Scholars of Liaoning Province[LJQ2015064] ; Natural Science Foundation Master Plan of Liaoning Province[201602282]
WOS关键词	HYALURONIC-ACID ; CARTILAGE ; RABBIT ; CELLS ; JOINT ; APOPTOSIS ; ADHESION ; PEPTIDE ; PATHWAY ; BINDING
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000398759400045
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/272664]
专题	天然药物化学研究室
通讯作者	Wang, Fengshan; Ling, Peixue; Mei, Xifan
作者单位	1.Univ Minnesota, Hormel Inst, Austin, MN 55912 USA 2.Shandong Univ, Sch Pharmaceut Sci, Jinan 250101, Peoples R China; 3.Inst Biopharmaceut Shandong Prov, Postdoctoral Sci Res Workstat, Jinan 250101, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 5.Jinzhou Med Univ, Affiliated Hosp 1, Jinzhou 121001, Peoples R China;
推荐引用方式 GB/T 7714	Han, Guanying,Chen, Qixin,Liu, Fei,et al. Low molecular weight xanthan gum for treating osteoarthritis[J]. CARBOHYDRATE POLYMERS,2017,164:386-395.
APA	Han, Guanying.,Chen, Qixin.,Liu, Fei.,Cui, Zan.,Shao, Huarong.,...&Mei, Xifan.(2017).Low molecular weight xanthan gum for treating osteoarthritis.CARBOHYDRATE POLYMERS,164,386-395.
MLA	Han, Guanying,et al."Low molecular weight xanthan gum for treating osteoarthritis".CARBOHYDRATE POLYMERS 164(2017):386-395.