Protective effects of beta-dihydroagarofuran-type sesquiterpene against Abeta_(25-35)-induced neuronal apoptosis and oxidative damage

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	Protective effects of beta-dihydroagarofuran-type sesquiterpene against Abeta_(25-35)-induced neuronal apoptosis and oxidative damage
	Ji SS(冀莎莎); Lei Y(雷芸); Huang XT(黄霄天); Gao ZQ(高志芹)
刊名	Journal of Chinese Pharmaceutical Sciences
	2016
卷号	25 期号:8 页码:582-589
关键词	Alzheimer's disease Abeta Neuroprotection Apoptosis Oxidative stress
ISSN号	1003-1057
其他题名	beta-二氢沉香呋喃倍半萜抗Abeta_(25-35)诱导的神经细胞凋亡和氧化损伤的保护作用
文献子类	Article
英文摘要	Excessive beta-amyloid (Abeta) plays a detrimental role in the pathogenesis of Alzheimer's disease (AD), which is closely associated with apoptosis and oxidative stress in neurons. Therefore, identification of active small molecules with potent effects on neutralizing Abeta-induced neurotoxicity would be a promising strategy for delaying or preventing AD progression. In the present study, we discovered that pretreatment with CF-1 ((1R,2S,4R,5S,7R,9S,10S)-1,15-diacetoxy-2-benzoyloxy-9-cinnamoyloxy- beta-di-hydroagarofuran), a sesquiterpene isolated from the seeds of Celastrus flagellaris, attenuated Abeta_(25-35)-induced reduction in cell viability in a concentration-dependent manner, as evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Above neuroprotective effect of CF-1 was associated with a significant decrease of apoptotic cells as measured by 4,6-diamidino-2-phenylindole (DAPI) staining, which concurrently happened with marked inhibition in the level of cleaved Caspase-3, an apoptotic executive protein. CF-1 pretreatment also markedly reduced the intracellular accumulation of reactive oxygen species (ROS) following Abeta exposure in SH-SY5Y neuroblastoma cells, but such pretreatment had no notable influence on 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging. In conclusion, we demonstrated that a novel natural product, CF-1, possessed promising effects against Abeta-induced neuronal apoptosis and oxidative stress, which could be a potential drug lead or candidate for the treatment of Abeta-associated neurotoxicity.
资助项目	National Natural Science Foundation of China[00000000]
WOS研究方向	Pharmacology & Pharmacy (provided by Clarivate Analytics)
语种	英语
CSCD记录号	CSCD:5786684
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/269239]
专题	药理学第二研究室
通讯作者	Huang XT(黄霄天)
作者单位	1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, CAS Key Laboratory of Receptor Research, Shanghai 201203, China. 2.School of Biological Science and Technology, Weifang Medical University, Weifang, Shandong 261053, China.;
推荐引用方式 GB/T 7714	Ji SS,Lei Y,Huang XT,et al. Protective effects of beta-dihydroagarofuran-type sesquiterpene against Abeta_(25-35)-induced neuronal apoptosis and oxidative damage[J]. Journal of Chinese Pharmaceutical Sciences,2016,25(8):582-589.
APA	冀莎莎,雷芸,黄霄天,&高志芹.(2016).Protective effects of beta-dihydroagarofuran-type sesquiterpene against Abeta_(25-35)-induced neuronal apoptosis and oxidative damage.Journal of Chinese Pharmaceutical Sciences,25(8),582-589.
MLA	冀莎莎,et al."Protective effects of beta-dihydroagarofuran-type sesquiterpene against Abeta_(25-35)-induced neuronal apoptosis and oxidative damage".Journal of Chinese Pharmaceutical Sciences 25.8(2016):582-589.