Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice

doi:10.3389/fphys.2018.00939

	Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice
	Gao, Fei 2; Liu, Xing 1,3; Shen, Ziying 2; Jia, Xiaohui 2; He, Han 1,3; Gao, Jing 1,3; Wu, Jianhong 1,3; Jiang, Chunhong 4; Zhou, Hu1,3 ; Wang, Yiping2
刊名	FRONTIERS IN PHYSIOLOGY
	2018-08-17
卷号	9
关键词	andrographolide sulfonate acute lung injury iTRAQ quantitative proteomics immunohistochemistry
ISSN号	1664-042X
DOI	10.3389/fphys.2018.00939
文献子类	Article
英文摘要	Andrographolide sulfonate (Andro-S), a sulfonation derivative of andrographolide, is known to be effective in treating inflammation-related diseases, while the underlying mechanisms and global protein alterations in response to Andro-S remain unknown. This study aimed to investigate the pharmacological effects and potential targets of Andro-S in a murine model of acute lung injury (ALI). ALI was induced by aerosolized lipopolysaccharide (LPS) exposure before treatment with Andro-S. Inflammatory state of each treatment group was determined by histological analysis and quantification of inflammatory markers. Differentially expressed proteins in lung tissues were identified by an iTRAQ-based quantitative proteomic approach and further confirmed by immunohistochemistry analysis. Administration of Andro-S alleviated LPS-induced histological changes in the lung and reduced the expression of inflammatory markers in serum, bronchoalveolar fluid and lung tissues. Proteomic analysis identified 31 differentially expressed proteins from a total of 2,234 quantified proteins in the lung. According to bioinformatics analysis, neutrophil elastase (ELANE), cathepsin G (CTSG) and myeloperoxidase (MPO), three neutrophil-derived proteases related to immune system process and defense responses to fungi were chosen as potential targets of Andro-S. Further immunohistochemistry analysis confirmed the inhibitory effects of Andro-S on LPS-induced ELANE, CTSG and MPO up-regulation. These results indicate that Andro-S suppressed the severity of LPS-induced ALI, possibly by attenuating the expression of and neutrophil-derived proteases.
资助项目	National Natural Science Foundation of China[31500110] ; National Natural Science Foundation of China[21375138] ; National Natural Science Foundation of China[31570830] ; Open Project Program of State Key Laboratory of Innovative Natural Medicine and TCM Injections[QFSKL2017005]
WOS关键词	SPECTROMETRY-BASED PROTEOMICS ; MOUTH-DISEASE HFMD ; MASS-SPECTROMETRY ; ORAL BIOAVAILABILITY ; CATHEPSIN-G ; IN-VITRO ; KAPPA-B ; PROTEIN ; QUANTIFICATION ; IDENTIFICATION
WOS研究方向	Physiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000441950700001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279616]
专题	分析化学研究室中科院受体结构与功能重点实验室新药研究国家重点实验室药理学第一研究室
通讯作者	Zhou, Hu; Wang, Yiping
作者单位	1.Univ Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Shanghai, Peoples R China; 2.Univ Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China; 3.Univ Chinese Acad Sci, Shanghai Inst Mat Med, Dept Analyt Chem, Shanghai, Peoples R China; 4.State Key Lab Innovat Nat Med & TCM Inject, Ganzhou, Peoples R China
推荐引用方式 GB/T 7714	Gao, Fei,Liu, Xing,Shen, Ziying,et al. Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice[J]. FRONTIERS IN PHYSIOLOGY,2018,9.
APA	Gao, Fei.,Liu, Xing.,Shen, Ziying.,Jia, Xiaohui.,He, Han.,...&Wang, Yiping.(2018).Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice.FRONTIERS IN PHYSIOLOGY,9.
MLA	Gao, Fei,et al."Andrographolide Sulfonate Attenuates Acute Lung Injury by Reducing Expression of Myeloperoxidase and Neutrophil-Derived Proteases in Mice".FRONTIERS IN PHYSIOLOGY 9(2018).