beta-Galactosidase BMG without galactose and glucose inhibition: Secretory expression in Bacillus subtilis and for synthesis of oligosaccharide

doi:10.1016/j.ijbiomac.2018.07.148

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	beta-Galactosidase BMG without galactose and glucose inhibition: Secretory expression in Bacillus subtilis and for synthesis of oligosaccharide
	Zhao, Lu 1; Zhou, Youzhi 1; Qin, Song 1,3; Qin, Panpan 1; Chu, Jianlin 2; He, Bingfang 1,2
刊名	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
	2018-12
卷号	120 页码:274-278
关键词	beta-Galactosidase Extracellular expression Galacto-oligosaccharides synthesis
ISSN号	0141-8130
DOI	10.1016/j.ijbiomac.2018.07.148
文献子类	Article
英文摘要	beta-Galactosidases can catalyze the hydrolysis of lactose and the synthesis of galacto-oligosaccharides (GOS) from lactose. The catalytic activity of13-galactosidases is usually inhibited by galactose and glucose, which hampers the complete hydrolysis of lactose in food products. In this report, a beta-galactosidase (denoted as BMG) from Bacillus megaterium YZO8 without the inhibition by galactose and glucose is the first to our knowledge reported. Efficient secretory expression of BMG was successfully achieved in Bacillus subtilis WB800. With the increasing of galactose and glucose, the activity of BMG dramatically enhanced. With addition of 0.6 M galactose or 1 M glucose, the initial activity to oNPG hydrolysis was increased by 2.1-fold and 4.3-fold, respectively. Moreover, the synthetic rate of galacto-oligosaccharides was enhanced by 1.1-fold and 1.9-fold with the addition of 0.7 M galactose or glucose, respectively. The GOS (278 g/L) from 600 g/L lactose by BMG were efficiently produced within 12 h, and the substrate lactose was completely used up. These results suggest that BMG shows a potential application in the food industry. (C) 2018 Elsevier B.V. All rights reserved.
资助项目	National Natural Science Foundation of China[81673321] ; National Natural Science Foundation of China[21506099] ; State Key Laboratory of Drug Research[SIMM1705KF-13] ; Natural Science Foundation of Jiangsu Province[BK20151541] ; Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture[XTC1812]
WOS关键词	BIOCHEMICAL-CHARACTERIZATION ; KLUYVEROMYCES-LACTIS ; LACTOSE ; HYDROLYSIS ; IMMOBILIZATION ; ENZYME
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000452587500032
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279475]
专题	上海中药现代化研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	He, Bingfang
作者单位	1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 Puzhunan Rd, Nanjing 211816, Jiangsu, Peoples R China; 2.Nanjing Tech Univ, Sch Pharmaceut Sci, 30 Puzhunan Rd, Nanjing 211816, Jiangsu, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Lu,Zhou, Youzhi,Qin, Song,et al. beta-Galactosidase BMG without galactose and glucose inhibition: Secretory expression in Bacillus subtilis and for synthesis of oligosaccharide[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,120:274-278.
APA	Zhao, Lu,Zhou, Youzhi,Qin, Song,Qin, Panpan,Chu, Jianlin,&He, Bingfang.(2018).beta-Galactosidase BMG without galactose and glucose inhibition: Secretory expression in Bacillus subtilis and for synthesis of oligosaccharide.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,120,274-278.
MLA	Zhao, Lu,et al."beta-Galactosidase BMG without galactose and glucose inhibition: Secretory expression in Bacillus subtilis and for synthesis of oligosaccharide".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 120(2018):274-278.