Apoferritin nanocages loading mertansine enable effective eradiation of cancer stem-like cells in vitro

doi:10.1016/j.ijpharm.2018.10.038

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	Apoferritin nanocages loading mertansine enable effective eradiation of cancer stem-like cells in vitro
	Tan, Tao 1; Wang, Yuqi 1; Wang, Hong 2,3; Cao, Haiqiang 2,3; Wang, Zhiwan 2,3; Wang, Jing 2,3; Li, Jie 2,3; Li, Yaping2,3 ; Zhang, Zhiwen2,3 ; Wang, Siling 1
刊名	INTERNATIONAL JOURNAL OF PHARMACEUTICS
	2018-12-20
卷号	553 期号:1-2 页码:201-209
关键词	Apoferritin Mertansine Cancer stem cells Breast cancer Nanocages Drug delivery
ISSN号	0378-5173
DOI	10.1016/j.ijpharm.2018.10.038
文献子类	Article
英文摘要	Cancer stem-like cells (CSCs) are proposed to be responsible for tumor metastasis, resistance and relapse after therapy, but are unable to be eliminated by many current therapies. Herein, we report that the apoferritin nanocages loading cytotoxic mertansine (M-AFN) can significantly improve their uptake in CSCs-enriched tumorspheres and effectively eradicate CSCs in tumorspheres for anticancer therapy. M-AFN were uniformly nanocage structures with the mean diameter of 11.26 +/- 2.58 nm and the loading capacity of 0.62%. In the CSCs-enriched tumorsphere model, M-AFN could be preferentially internalized by tumorsphere cells and the average half-inhibitory concentration (IC50) of M-AFN was obviously reduced by 5.46-fold when comparing to the parent 4T1 breast cancer cells. Moreover, both the already existing tumorspheres and the formation of secondary tumorspheres were drastically disrupted by M-AFN, but barely impacted by mertansine alone. The flow cytometer analysis showed the CSCs fractions in tumorspheres were considerably reduced by the M-AFN treatment. Therefore, the apoferritin nanocages represent an encouraging nanoplatform to eradicate CSCs for effective anticancer therapy.
资助项目	National Basic Research Program of China[2015CB932103] ; National Natural Science Foundation of China[31771092] ; National Natural Science Foundation of China[81521005] ; National Natural Science Foundation of China[81690265] ; Youth Innovation Promotion Association of Chinese Academy of Sciences[00000000]
WOS关键词	FERRITIN NANOCAGES ; LUNG METASTASIS ; TUMOR ; DELIVERY ; THERAPY ; NANOPARTICLES ; NANOMEDICINES ; DOXORUBICIN ; GLIOBLASTOMA ; FORMULATION
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000451020300019
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279465]
专题	药物制剂研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zhang, Zhiwen; Wang, Siling
作者单位	1.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Liaoning, Peoples R China; 2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Chinese Acad Sci, Ctr Pharmaceut, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Tan, Tao,Wang, Yuqi,Wang, Hong,et al. Apoferritin nanocages loading mertansine enable effective eradiation of cancer stem-like cells in vitro[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2018,553(1-2):201-209.
APA	Tan, Tao.,Wang, Yuqi.,Wang, Hong.,Cao, Haiqiang.,Wang, Zhiwan.,...&Wang, Siling.(2018).Apoferritin nanocages loading mertansine enable effective eradiation of cancer stem-like cells in vitro.INTERNATIONAL JOURNAL OF PHARMACEUTICS,553(1-2),201-209.
MLA	Tan, Tao,et al."Apoferritin nanocages loading mertansine enable effective eradiation of cancer stem-like cells in vitro".INTERNATIONAL JOURNAL OF PHARMACEUTICS 553.1-2(2018):201-209.