A Theoretical Model for Calculating Voltage Sensitivity of Ion Channels and the Application on Kv1.2 Potassium Channel

doi:10.1016/j.bpj.2012.03.032

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	A Theoretical Model for Calculating Voltage Sensitivity of Ion Channels and the Application on Kv1.2 Potassium Channel
	Yang, Huaiyu 1; Gao, Zhaobing1 ; Li, Ping 1; Yu, Kunqian1 ; Yu, Ye 2; Xu, Tian-Le 2; Li, Min 3,4; Jiang, Hualiang1
刊名	BIOPHYSICAL JOURNAL
	2012-04-18
卷号	102 期号:8 页码:1815-1825
ISSN号	0006-3495
DOI	10.1016/j.bpj.2012.03.032
文献子类	Article
英文摘要	Voltage sensing confers conversion of a change in membrane potential to signaling activities underlying the physiological processes. For an ion channel, voltage sensitivity is usually experimentally measured by fitting electrophysiological data to Boltzmann distributions. In our study, a two-state model of the ion channel and equilibrium statistical mechanics principle were used to test the hypothesis of empirically calculating the overall voltage sensitivity of an ion channel on the basis of its closed and open conformations, and determine the contribution of individual residues to the voltage sensing. We examined the theoretical paradigm by performing experimental measurements with Kv1.2 channel and a series of mutants. The correlation between the calculated values and the experimental values is at respective level, R-2 = 0.73. Our report therefore provides in silico prediction of key conformations and has identified additional residues critical for voltage sensing.
资助项目	State Key Program of Basic Research of China[2009CB918502] ; National Natural Science Foundation of China[81173027] ; National Natural Science Foundation of China[81072579] ; National Natural Science Foundation of China[21021063] ; National Institutes of Health[MH084691]
WOS关键词	SHAKER K+ CHANNEL ; MOLECULAR-DYNAMICS SIMULATIONS ; SODIUM-CHANNELS ; GATING-CHARGE ; ELECTROSTATIC INTERACTIONS ; S4-S5 LINKER ; TRANSMEMBRANE SEGMENTS ; SENSING RESIDUES ; SENSOR MOVEMENT ; ELECTRIC-FIELD
WOS研究方向	Biophysics
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000303003300015
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278114]
专题	神经药理学研究国际科学家工作站中科院受体结构与功能重点实验室新药研究国家重点实验室药物发现与设计中心
通讯作者	Jiang, Hualiang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200030, Peoples R China; 3.Johns Hopkins Univ, High Throughput Biol Ctr, Dept Neurosci, Baltimore, MD USA; 4.Johns Hopkins Univ, Sch Med, Johns Hopkins Ion Channel Ctr, Baltimore, MD USA
推荐引用方式 GB/T 7714	Yang, Huaiyu,Gao, Zhaobing,Li, Ping,et al. A Theoretical Model for Calculating Voltage Sensitivity of Ion Channels and the Application on Kv1.2 Potassium Channel[J]. BIOPHYSICAL JOURNAL,2012,102(8):1815-1825.
APA	Yang, Huaiyu.,Gao, Zhaobing.,Li, Ping.,Yu, Kunqian.,Yu, Ye.,...&Jiang, Hualiang.(2012).A Theoretical Model for Calculating Voltage Sensitivity of Ion Channels and the Application on Kv1.2 Potassium Channel.BIOPHYSICAL JOURNAL,102(8),1815-1825.
MLA	Yang, Huaiyu,et al."A Theoretical Model for Calculating Voltage Sensitivity of Ion Channels and the Application on Kv1.2 Potassium Channel".BIOPHYSICAL JOURNAL 102.8(2012):1815-1825.