Chemical and biochemical approaches in the study of histone methylation and demethylation

doi:10.1002/med.20228

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物发现与设计中心

	Chemical and biochemical approaches in the study of histone methylation and demethylation
	Li, Keqin Kathy 1,4; Luo, Cheng2 ; Wang, Dongxia 5; Jiang, Hualiang2 ; Zheng, Y. George 3
刊名	MEDICINAL RESEARCH REVIEWS
	2012-07
卷号	32 期号:4 页码:815-867
关键词	histone methylation demethylation ChIP mass spectrometry chemical ligation inhibitor
ISSN号	0198-6325
DOI	10.1002/med.20228
文献子类	Review
英文摘要	Histone methylation represents one of the most critical epigenetic events in DNA function regulation in eukaryotic organisms. Classic molecular biology and genetics tools provide significant knowledge about mechanisms and physiological roles of histone methyltransferases and demethylases in various cellular processes. In addition to this stream line, development and application of chemistry and chemistry-related techniques are increasingly involved in biological study, and offer information otherwise difficult to obtain by standard molecular biology methods. Herein, we review recent achievements and progress in developing and applying chemical and biochemical approaches in the study of histone methylation, including chromatin immunoprecipitation, chemical ligation, mass spectrometry, biochemical methylation and demethylation assays, and inhibitor development. These technological advances allow histone methylation to be studied from genome-wide level to molecular and atomic levels. With ChIP technology, information can be obtained about precise mapping of histone methylation patterns at specific promoters, genes, or other genomic regions. MS is particularly useful in detecting and analyzing methylation marks in histone and nonhistone protein substrates. Chemical approaches that permit site-specific incorporation of methyl groups into histone proteins greatly facilitate the investigation of biological impacts of methylation at individual modification sites. Discovery and design of selective organic inhibitors of histone methyltransferases and demethylases provide chemical probes to interrogate methylation-mediated cellular pathways. Overall, these chemistry-related technological advances have greatly improved our understanding of the biological functions of histone methylation in normal physiology and diseased states, and also are of great potential to translate basic epigenetics research into diagnostic and therapeutic applications in the clinic. (C) 2010 Wiley Periodicals, Inc. Med Res Rev., 32, No. 4, 815-867, 2012
资助项目	National Natural Science Foundation of China[20972174] ; Shanghai Science and Technology Innovation Program[10410703900] ; State Key Program of Basic Research of China[2009CB918502] ; National ST Major Project[2009ZX09501-001] ; Georgia Cancer Coalition Distinguished Cancer Scientist program[00000000] ; American Heart Association[00000000]
WOS关键词	TANDEM MASS-SPECTROMETRY ; PROTEIN-ARGININE-METHYLTRANSFERASE ; ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES ; TIME-OF-FLIGHT ; LYSINE 36-SPECIFIC METHYLTRANSFERASE ; CONTINUOUS SPECTROPHOTOMETRIC ASSAY ; ELECTRON-CAPTURE DISSOCIATION ; MECHANISM-BASED INACTIVATOR ; EMBRYONIC STEM-CELLS ; POSTTRANSLATIONAL MODIFICATIONS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000306140000006
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/278026]
专题	药物发现与设计中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zheng, Y. George
作者单位	1.US SINO Epigenet Assoc, Norcross, GA USA; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Shanghai 200031, Peoples R China; 3.Georgia State Univ, Dept Chem, Atlanta, GA 30302 USA; 4.Shanghai Jiao Tong Univ, Sch Med, Rui Jin Hosp, State Key Lab Med Genom,Shanghai Inst Hematol, Shanghai 200030, Peoples R China; 5.Ctr Dis Control & Prevent CDC, Biol Mass Spectrometry Lab, Atlanta, GA USA
推荐引用方式 GB/T 7714	Li, Keqin Kathy,Luo, Cheng,Wang, Dongxia,et al. Chemical and biochemical approaches in the study of histone methylation and demethylation[J]. MEDICINAL RESEARCH REVIEWS,2012,32(4):815-867.
APA	Li, Keqin Kathy,Luo, Cheng,Wang, Dongxia,Jiang, Hualiang,&Zheng, Y. George.(2012).Chemical and biochemical approaches in the study of histone methylation and demethylation.MEDICINAL RESEARCH REVIEWS,32(4),815-867.
MLA	Li, Keqin Kathy,et al."Chemical and biochemical approaches in the study of histone methylation and demethylation".MEDICINAL RESEARCH REVIEWS 32.4(2012):815-867.