The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus

doi:10.1038/aps.2013.3

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药理学第二研究室

	The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus
	Wang, Jun 2; Wang, Yu-hua 2,3; Hou, Yuan-yuan 1; Xi, Tao 2; Liu, Yao 1; Liu, Jing-gen1
刊名	ACTA PHARMACOLOGICA SINICA
	2013-06
卷号	34 期号:6 页码:811-818
关键词	morphine withdrawal aversive memory conditioned place aversion dorsal hippocampus actin rearrangement RhoA Rac1 naloxone Y27632 NSC23766
ISSN号	1671-4083
DOI	10.1038/aps.2013.3
文献子类	Article
英文摘要	Aim: Actin rearrangements are induced in the dorsal hippocampus after conditioned morphine withdrawal, and involved in the formation of conditioned place aversion. In the present study, we investigated the mechanisms underlying the actin rearrangements in rat dorsal hippocampus induced by conditioned morphine withdrawal. Methods: The RhoA-ROCK pathway inhibitor Y27632 (8.56 mu g/1 mu L per side) or the Rac1 inhibitor NSC23766 (25 mu g/1 mu L per side) was microinjected into the dorsal hippocampus of rats. Conditioned place aversion (CPA) induced by naloxone-precipitated morphine withdrawal was assessed. Crude synaptosomal fraction of hippocampus was prepared, and the amount of F-actin and G-actin was measured with an Actin Polymerization Assay Kit. Results: Conditioned morphine withdrawal significantly increased actin polymerization in the dorsal hippocampus at 1 h following the naloxone injection. Preconditioning with microinjection of Y27632, but not NSC23766, attenuated CPA, and blocked the increase in actin polymerization in the dorsal hippocampus. Conclusion: Our results suggest that the small GTPase RhoA, but not Rac1, in the dorsal hippocampus is responsible for CPA formation, mainly through its regulation of actin rearrangements.
资助项目	Ministry of Science and Technology of China[2009CB522005] ; Foundation of National Natural Science of China[81130087] ; Foundation of National Natural Science of China[81001424] ; Foundation of Natural Science of Jiangshu Province[SBK201241521]
WOS关键词	TERM SYNAPTIC PLASTICITY ; DENDRITIC SPINES ; STRUCTURAL PLASTICITY ; DRUG-WITHDRAWAL ; CYTOSKELETON ; DYNAMICS ; FAMILY ; POLYMERIZATION ; MAINTENANCE ; EXPRESSION
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:4850358
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000319915000013
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277597]
专题	药理学第二研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Xi, Tao
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.China Pharmaceut Univ, Sch Life Sci & Technol, Ctr Biotechnol, Nanjing 210009, Jiangsu, Peoples R China; 3.Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210046, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jun,Wang, Yu-hua,Hou, Yuan-yuan,et al. The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus[J]. ACTA PHARMACOLOGICA SINICA,2013,34(6):811-818.
APA	Wang, Jun,Wang, Yu-hua,Hou, Yuan-yuan,Xi, Tao,Liu, Yao,&Liu, Jing-gen.(2013).The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus.ACTA PHARMACOLOGICA SINICA,34(6),811-818.
MLA	Wang, Jun,et al."The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus".ACTA PHARMACOLOGICA SINICA 34.6(2013):811-818.