Simultaneous determination of ornidazole and its main metabolites in human plasma by LC-MS/MS: application to a pharmacokinetic study

doi:10.4155/bio.14.117

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 上海药物代谢研究中心

	Simultaneous determination of ornidazole and its main metabolites in human plasma by LC-MS/MS: application to a pharmacokinetic study
	Du, Jiangbo 1; Ma, Zhiyu 1; Zhang, Yifan1 ; Wang, Ting 2; Chen, Xiaoyan1 ; Zhong, Dafang1
刊名	BIOANALYSIS
	2014
卷号	6 期号:18 页码:2343-2356
ISSN号	1757-6180
DOI	10.4155/bio.14.117
文献子类	Article
英文摘要	Background: Ornidazole is a 5-nitroimidazole antimicrobial agent used for almost 40 years. A novel LC-MS/MS assay was developed and validated for the simultaneous determination of ornidazole and its main metabolites (M3, M6, M16-1, and M16-2) in human plasma. Results: After extraction from 100 l of plasma by protein precipitation with acetonitrile, all the analytes were separated on a Capcell PAK MG C-18 column (100 x 4.6 mm, 5 m) within 5.0 min and detected by ESI-MS/MS in the positive mode. The validation results met the acceptance criteria as per the US FDA and EMA guidelines. Conclusion: The validated method was successfully applied to a pharmacokinetic study after oral administration of 1000 mg ornidazole to six healthy Chinese volunteers.
WOS关键词	PERFORMANCE LIQUID-CHROMATOGRAPHY ; INCURRED SAMPLE REANALYSIS ; TANDEM MASS-SPECTROMETRY ; GLOBAL CRO COUNCIL ; ANAEROBIC-BACTERIA ; EPOXIDE HYDROLASES ; BIOLOGICAL-FLUIDS ; METRONIDAZOLE ; MECHANISM ; NITROIMIDAZOLES
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	FUTURE SCI LTD
WOS记录号	WOS:000349184300004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277246]
专题	上海药物代谢研究中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Zhong, Dafang
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Lanzhou Univ, Affiliated Hosp 1, Lanzhou 730000, Peoples R China
推荐引用方式 GB/T 7714	Du, Jiangbo,Ma, Zhiyu,Zhang, Yifan,et al. Simultaneous determination of ornidazole and its main metabolites in human plasma by LC-MS/MS: application to a pharmacokinetic study[J]. BIOANALYSIS,2014,6(18):2343-2356.
APA	Du, Jiangbo,Ma, Zhiyu,Zhang, Yifan,Wang, Ting,Chen, Xiaoyan,&Zhong, Dafang.(2014).Simultaneous determination of ornidazole and its main metabolites in human plasma by LC-MS/MS: application to a pharmacokinetic study.BIOANALYSIS,6(18),2343-2356.
MLA	Du, Jiangbo,et al."Simultaneous determination of ornidazole and its main metabolites in human plasma by LC-MS/MS: application to a pharmacokinetic study".BIOANALYSIS 6.18(2014):2343-2356.