MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1

doi:10.1038/aps.2016.92

CORC > 上海药物研究所 > 中国科学院上海药物研究所 > 药物安全性评价中心

	MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1
	Li, Cheng-gang 1,2; Pu, Meng-fan 1,2; Li, Chun-zhu 1,2; Gao, Man 1,2; Liu, Ming-xia 1,2; Yu, Cun-zhi 1,2; Yan, Hong 1,2; Peng, Chun 1,2; Zhao, Yang 1,2; Li, Yu 1,2
刊名	ACTA PHARMACOLOGICA SINICA
	2017-01
卷号	38 期号:1 页码:110-119
关键词	miR-1304 NSCLC heme oxygenase-1 hemin
ISSN号	1671-4083
DOI	10.1038/aps.2016.92
文献子类	Article
英文摘要	Previous studies have shown that microRNA-1304 (miR-1304) is dysregulated in certain types of cancers, including non-small cell lung cancer (NSCLC), and might be involved in tumor survival and/or growth. In this study we investigated the direct target of miR-1304 and its function in NSCLC in vitro. Human lung adenocarcinoma cell lines (A549 and NCI-H1975) were studied. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays. Cell apoptosis and cell cycle were examined using annexin V-PE/7-AAD and PI staining assays, respectively. The dual-luciferase reporter assay was used to verify post-transcriptional regulation of heme oxygenase-1 (HO-1) by miR-1304. CRISPR/Cas9 was used to deplete endogenous miR-1304. Overexpression of MiR-1304 significantly decreased the number and viability of NSCLC cells and colony formation, and induced cell apoptosis and G(0)/G(1) phase cell cycle arrest. HO-1 was demonstrated to be a direct target of miR-1304 in NSCLC cells. Restoration of HO-1 expression by hemin (20 mu mol/L) abolished the inhibition of miR-1304 on cell growth and rescued miR-1304-induced apoptosis in A549 cells. Suppression of endogenous miR-1304 with anti-1304 significantly increased HO-1 expression and promoted cell growth and survival in A549 cells. In 17 human NSCLC tissue samples, miR-1304 expression was significantly decreased, while HO-1 expression was significantly increased as compared to normal lung tissues. MicroRNA-1304 is a tumor suppressor and HO-1 is its direct target in NSCLC. The results suggest the potential for miR-1304 as a therapeutic target for NSCLC.
资助项目	National Science and Technology Major Project[2012ZX09302-003] ; National Science and Technology Major Project[2012ZX09301-001-006] ; National Science and Technology Major Project[2015ZX09102005]
WOS关键词	BREAST-CANCER ; PROLIFERATION ; EXPRESSION ; PATHWAY ; HO-1 ; TRANSLATION ; METASTASIS ; INHIBITION ; CARCINOMA ; APOPTOSIS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:5906822
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000393540900010
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/275741]
专题	药物安全性评价中心中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Miao, Ling-ling; Ren, Jin
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China; 2.Univ Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Ctr Drug Safety Evaluat & Res, Beijing 100049, Peoples R China; 3.Beijing Inst Basic Med Sci, Brain Sci Ctr, Beijing 100850, Peoples R China
推荐引用方式 GB/T 7714	Li, Cheng-gang,Pu, Meng-fan,Li, Chun-zhu,et al. MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1[J]. ACTA PHARMACOLOGICA SINICA,2017,38(1):110-119.
APA	Li, Cheng-gang.,Pu, Meng-fan.,Li, Chun-zhu.,Gao, Man.,Liu, Ming-xia.,...&Ren, Jin.(2017).MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1.ACTA PHARMACOLOGICA SINICA,38(1),110-119.
MLA	Li, Cheng-gang,et al."MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1".ACTA PHARMACOLOGICA SINICA 38.1(2017):110-119.