Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro

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	Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro
	Wu, SF ; Sun, HZ ; Tu, ZH ; Wu, HY
刊名	ACTA PHARMACOLOGICA SINICA
	2001-03
卷号	22 期号:3 页码:257-263
关键词	epristeride prostate epithelium cultured cells epidermal growth factor receptors IGF type I receptor reverse polymerase chain reaction flow cytometry
ISSN号	0253-9756
文献子类	Article
英文摘要	AIM: To study the molecular mechanism of rat prostate atrophy induced by epristeride. METHODS: MTT test was used to determine the effect of epristeride on the growth of prostatic epithelial cell induced by exogenous epithelial growth factor (EGF) or insulin-like growth factor-I (IGF-I). RT-PCR and flow cytometry were then used to quantitatively detect the mRNA and protein expressions of EGFR and IGF-I R of the epithelial cells treated or untreated with epristeride. RESULTS: Epristeride attenuated growth of epithelial cells induced by exogenous EGF, IGF-I. Epristeride 360 nmol/L inhibited EGFR and IGF-I R expression at mRNA level, while epristeride 180 nmol/L had no marked effect on EGFR and IGF-I R mRNA expression. Both epristeride 180 nmol/L and 360 nmol/L could down regulate EGFR and IGF-IR protein levels. CONCLUSION: The molecular mechanisms of prostatic epithelial cell atrophy induced by epristeride might be associated with alteration in the expression of growth factor receptors such as EGF and IGF-I.
WOS关键词	IGF-BINDING PROTEINS ; FACTOR-I ; VENTRAL PROSTATE ; FACTOR-ALPHA ; 5-ALPHA-REDUCTASE ; FINASTERIDE ; RECEPTOR ; TISSUE ; LOCALIZATION ; HYPERPLASIA
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
出版者	ACTA PHARMACOLOGICA SINICA
WOS记录号	WOS:000167403800011
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274513]
专题	新药研究国家重点实验室中科院受体结构与功能重点实验室
通讯作者	Tu, ZH
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Fudan Univ, Med Ctr, Shanghai Peoples Hosp 1, Dept Urol, Shanghai 200032, Peoples R China
推荐引用方式 GB/T 7714	Wu, SF,Sun, HZ,Tu, ZH,et al. Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro[J]. ACTA PHARMACOLOGICA SINICA,2001,22(3):257-263.
APA	Wu, SF,Sun, HZ,Tu, ZH,&Wu, HY.(2001).Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro.ACTA PHARMACOLOGICA SINICA,22(3),257-263.
MLA	Wu, SF,et al."Inhibition of cultured rat prostatic epithelial cell growth by epristeride in vitro".ACTA PHARMACOLOGICA SINICA 22.3(2001):257-263.