In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS

doi:10.1016/j.bpc.2004.06.008

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	In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS
	Luo, HB ; Ye, F ; Sun, T ; Yue, LD ; Peng, SY ; Chen, J; Li, GW ; Du, Y ; Xie, YH ; Yang, YM
刊名	BIOPHYSICAL CHEMISTRY
	2004-12-01
卷号	112 期号:1 页码:15-25
关键词	SARS nucleocapsid protein SPR circular dichmism fluorescence protein folding and unfolding
ISSN号	0301-4622
DOI	10.1016/j.bpc.2004.06.008
文献子类	Article
英文摘要	The major biochemical and thermodynamic features of nucelocapsid protein of SARS coronavirus (SARS-NP) were characterized by use of non-denatured gel electrophoresis, size-exclusion chromatographic and surface plasmon resonance (SPR) techniques. The results showed that SARS_NP existed in vitro as oligomer, more probably dimer, as the basic functional unit. This protein shows its maximum conformational stability near pH 9.0, and it seems that its oligomer dissociation and protein unfolding occur simultaneously. Thermal-induced unfolding for SARS_NP was totally irreversible. Both the thermal and chemical denaturant-induced denaturation analyses showed that oligomeric SARS_NP unfolds and refolds through a two-state model, and the electrostatic interactions among the charge groups of SARS_NP made a significant contribution to its conformational stability. (C) 2004 Elsevier B.V. All rights reserved.
WOS关键词	ACUTE RESPIRATORY SYNDROME ; MOUSE HEPATITIS-VIRUS ; ENVELOPE PROTEIN ; ESCHERICHIA-COLI ; P-PROTEIN ; CORONAVIRUS ; RNA ; STABILITY ; IDENTIFICATION ; PURIFICATION
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Chemistry
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000224852300003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273982]
专题	药理学第三研究室中科院受体结构与功能重点实验室新药研究国家重点实验室药物发现与设计中心
通讯作者	Shen, X
作者单位	1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Drug Discovery & Design Ct, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Grad Sch, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Luo, HB,Ye, F,Sun, T,et al. In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS[J]. BIOPHYSICAL CHEMISTRY,2004,112(1):15-25.
APA	Luo, HB.,Ye, F.,Sun, T.,Yue, LD.,Peng, SY.,...&Jiang, HL.(2004).In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS.BIOPHYSICAL CHEMISTRY,112(1),15-25.
MLA	Luo, HB,et al."In vitro biochemical and thermodynamic characterization of nucleocapsid protein of SARS".BIOPHYSICAL CHEMISTRY 112.1(2004):15-25.