T-cell receptor (TCR)/CD3 is involved in sulfated polymannuroguluronate (SPMG)-induced T lymphocyte activation

doi:10.1016/j.intimp.2005.02.011

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	T-cell receptor (TCR)/CD3 is involved in sulfated polymannuroguluronate (SPMG)-induced T lymphocyte activation
	Miao, BC ; Li, J ; Fu, XY ; Ding, J; Geng, MY
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2005-07
卷号	5 期号:7-8 页码:1171-1182
关键词	SPMG T lymphocytes TCR/CD3 ConA inummopotentiating activities
ISSN号	1567-5769
DOI	10.1016/j.intimp.2005.02.011
文献子类	Article
英文摘要	Sulfated polymannuroguluronate (SPMG) has entered the Phase II clinical trial as the first anti-acquired immune deficiency syndrome (AIDS) drug candidate in China. Proliferation assays showed that SPMG was effective at enhancing the proliferative response of T lymphocytes either with or without concanavalin A (ConA) stimulation. Flow cytometry (FCM) and fluorescence microscope examination revealed the significant binding of SPMG to T lymphocytes. The significant engagement of SPMG with TCR/CD3 complex was verified by competitive inhibition assay and one of the SPMG binding proteins purified by affinity chromatography from thymocyte membrane preparations was further confirmed to be CD3 component of TCR/CD3 complex via Western blotting analysis. In addition, SPMG was demonstrated to dramatically interact with ConA in a multivalent manner by surface plasmon resonance (SPR) assay. Notably, the concomitant presence of ConA and SPMG facilitated each other's binding to T cells. Together, the simultaneous interactions of SPMG with TCR/CD3 and with ConA can be highly proposed to facilitate the cross-linking of these molecules, and thus favoring costimulatory signaling, which serves to well explain the immunopotentiation and anti-human immunodeficiency virus (HIV) activities of SPMG. (C) 2005 Elsevier B.V. All rights reserved.
WOS关键词	TYROSINE PHOSPHORYLATION ; SIGNAL-TRANSDUCTION ; CONCANAVALIN-A ; PROTEIN-KINASE ; DYE-BINDING ; IN-VITRO ; ANTIGEN ; TCR ; CD4 ; POLYSACCHARIDE
WOS研究方向	Immunology ; Pharmacology & Pharmacy
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000229714300007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/273847]
专题	药理学第一研究室中科院受体结构与功能重点实验室新药研究国家重点实验室
通讯作者	Ding, J
作者单位	1.Ocean Univ China, Marine Drug & Food Inst, Dept Pharmacol, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res,Div Antitumor Pharmacol, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Miao, BC,Li, J,Fu, XY,et al. T-cell receptor (TCR)/CD3 is involved in sulfated polymannuroguluronate (SPMG)-induced T lymphocyte activation[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2005,5(7-8):1171-1182.
APA	Miao, BC,Li, J,Fu, XY,Ding, J,&Geng, MY.(2005).T-cell receptor (TCR)/CD3 is involved in sulfated polymannuroguluronate (SPMG)-induced T lymphocyte activation.INTERNATIONAL IMMUNOPHARMACOLOGY,5(7-8),1171-1182.
MLA	Miao, BC,et al."T-cell receptor (TCR)/CD3 is involved in sulfated polymannuroguluronate (SPMG)-induced T lymphocyte activation".INTERNATIONAL IMMUNOPHARMACOLOGY 5.7-8(2005):1171-1182.