TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury

doi:10.1038/s41598-018-22852-2

	TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury
	Liu, Aifen 2; Wu, Jing 3; Yang, Cheng 4,5; Wu, Yuanyuan 6; Zhang, Yufang 2; Zhao, Fengbo 2; Wang, Hui 3; Yuan, Li 3; Song, Lirui 7,8; Zhu, Tongyu 4,5
刊名	SCIENTIFIC REPORTS
	2018-04-03
卷号	8
ISSN号	2045-2322
DOI	10.1038/s41598-018-22852-2
文献子类	Article
英文摘要	Transient receptor potential melastatin 7 (TRPM7) is a membrane ion channel and kinase. TRPM7 was abundantly expressed in the kidney, and up-regulated by ischemia reperfusion (IR) injury. Our previous studies showed that cyclic helix B peptide (CHBP) improved renal IR-related injury, but its underlying mechanism is not well defined. IR-related injury was established in renal tubular epithelial cells (TCMK-1 and HK-2) via 12 to 24-h hypoxia (H) followed by 2-24 h reoxygenation (R), and in mouse kidneys subjected to 30-min ischemia and 12-h to 7-day reperfusion. TRPM7-like current in TCMK-1 cells, TRPM7 mRNA and protein in the in vitro and in vivo models were increased, but reversed by CHBP. TRPM7 was also positively associated with LDH, HMGB1, caspase-3, Bax/Bcl-2, inflammation, apoptosis, tubulointerstitial damage and renal function respectively. Furthermore, silencing TRPM7 improved injury parameters, renal histology and function in the both models. Specific TRPM7 agonist, bradykinin, exaggerated HR induced injury in TCMK-1 cells, and partially blocked the renoprotection of CHBP as well. In conclusion, TRPM7 is involved not only in IR-related injury, but also CHBP-induced renoprotection, which are through its ion channel and subsequent affects inflammation and apoptosis. Therefore, TRPM7 could be a potential biomarker for IR-induced acute kidney injury.
资助项目	National Natural Science Foundation of China[81300564] ; National Natural Science Foundation of China[81570677] ; National Natural Science Foundation of China[81170689] ; National Natural Science Foundation of China[81400752] ; National Natural Science Foundation of China[81402189] ; National Natural Science Foundation of China[81270833] ; National Natural Science Foundation of China[81570674] ; Science and Technology Projects of Nantong[MS12015117] ; Natural Science Foundation of Jiangsu Province[BK20140437]
WOS关键词	RENAL ISCHEMIA/REPERFUSION INJURY ; POTENTIAL MELASTATIN 7 ; ACUTE KIDNEY INJURY ; HELIX B PEPTIDE ; CELL-DEATH ; NEURONAL DEATH ; RECEPTOR 4 ; RAT MODEL ; APOPTOSIS ; ERYTHROPOIETIN
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000428999200046
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/279812]
专题	中国科学院上海药物研究所
通讯作者	Yang, Bin
作者单位	1.Univ Leicester, Leicester Gen Hosp, Univ Hosp Leicester, Dept Infect Immun & Inflammat, Leicester LE1 9HN, Leics, England 2.Nantong Univ, Med Coll, Basic Med Res Ctr, Renal Grp, Nantong 226001, Jiangsu, Peoples R China; 3.Nantong Univ, Affiliated Hosp, Dept Nephrol, Nantong 226001, Jiangsu, Peoples R China; 4.Fudan Univ, Zhongshan Hosp, Dept Urol, Shanghai 200032, Peoples R China; 5.Shanghai Key Lab Organ Transplantat, Shanghai 200032, Peoples R China; 6.Nantong Univ, Med Coll, Dept Pathol, Nantong 226001, Jiangsu, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
推荐引用方式 GB/T 7714	Liu, Aifen,Wu, Jing,Yang, Cheng,et al. TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury[J]. SCIENTIFIC REPORTS,2018,8.
APA	Liu, Aifen.,Wu, Jing.,Yang, Cheng.,Wu, Yuanyuan.,Zhang, Yufang.,...&Yang, Bin.(2018).TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury.SCIENTIFIC REPORTS,8.
MLA	Liu, Aifen,et al."TRPM7 in CHBP-induced renoprotection upon ischemia reperfusion-related injury".SCIENTIFIC REPORTS 8(2018).