HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression

doi:10.1159/000354460

	HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression
	Shi, Chen-Ye 2; Fan, Yue 1; Liu, Bin 3; Lou, Wen-Hui 2
刊名	CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
	2013
卷号	32 期号:3 页码:561-568
关键词	Hypoxia HIF-1 QSOX1 Matrix Metalloproteinase Pancreatic Cancer Cell
ISSN号	1015-8987
DOI	10.1159/000354460
文献子类	Article
英文摘要	Background: Quiescin sulfhydryl oxidase 1 (QSOX1), which oxidizes sulfhydryl groups to form disulfide bonds in proteins, is found to be over-expressed in various pancreatic cancer cell lines and patients. QSOX1 promotes invasion of pancreatic cancer cells by activating MMP-2 and MMP-9. However, its regulatory mechanism remains largely undefined. Methods: Realtime PCR and Western blot were employed to detect the expression of QSOX1 in human pancreatic cancer cell lines under hypoxic condition. Luciferase reporter and ChIP assays were used to assess the regulation of QSOX1 by hypoxia-inducible factor 1 (HIF-1). Small interfering RNA (siRNA) was applied to knock down endogenous expression of QSOX1. Matrigel-coated invasion chamber essays were conducted to detect the invasion capacity of QSOX1-depleted cells. Results: Both hypoxia and hypoxia mimicking reagent up-regulated the expression of QSOX1 in human pancreatic cancer cell lines. Knockdown of HIF-1 alpha eliminated hypoxia induced QSOX1 expression. HIF-1a was found directly bound to two hypoxia-response elements (HRE) of QSOX1 gene, both of which were required for HIF-1 induced QSOX1 expression. Moreover, QSOX1 silencing blocked hypoxia-induced pancreatic cancer cells invasion. Conclusion: QSOX1 is a direct target of HIF-1 and may contribute to hypoxia-induced pancreatic cancer cells invasion.Copyright (C) 2013 S. Karger AG, Basel
WOS关键词	QUIESCIN-SULFHYDRYL OXIDASE ; THERAPY
WOS研究方向	Cell Biology ; Physiology
语种	英语
出版者	KARGER
WOS记录号	WOS:000325415900007
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/277787]
专题	中国科学院上海药物研究所
通讯作者	Lou, Wen-Hui
作者单位	1.Fudan Univ, Zhongshan Hosp, Dept Integrated TCM & Western Med, Shanghai 200032, Peoples R China; 2.Fudan Univ, Zhongshan Hosp, Dept Gen Surg, Shanghai 200032, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Shi, Chen-Ye,Fan, Yue,Liu, Bin,et al. HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2013,32(3):561-568.
APA	Shi, Chen-Ye,Fan, Yue,Liu, Bin,&Lou, Wen-Hui.(2013).HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,32(3),561-568.
MLA	Shi, Chen-Ye,et al."HIF1 Contributes to Hypoxia-Induced Pancreatic Cancer Cells Invasion via Promoting QSOX1 Expression".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 32.3(2013):561-568.