Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells

doi:10.1016/S0006-2952(99)00255-5

	Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells
	Cheung, F ; Siow, YL ; Chen, WZ ; O, K
刊名	BIOCHEMICAL PHARMACOLOGY
	1999-11-15
卷号	58 期号:10 页码:1665-1673
关键词	nitric oxide endothelial cells Ginkgo biloba leaf extracts
ISSN号	0006-2952
DOI	10.1016/S0006-2952(99)00255-5
文献子类	Article
英文摘要	Excessive production of nitric oxide (NO) may have cytotoxic effects through the formation of peroxynitrite with superoxide. The extract of Ginkgo biloba leaves (EGb) has been demonstrated to be a potent scavenger of free radicals. Although EGb has been shown recently to inhibit NO production in macrophages, its effect on NO production in endothelial cells is largely unknown. The objective of this study was to elucidate the mechanism by which EGb affects NO production in a human endothelial cell line (EGV304). After cells were incubated with EGb (10-100 mu g/mL) for 2 or 4 hr, the amounts of NO metabolites released by the cells were quantitated, and cellular NOS activities were determined following the conversion of [H-3]arginine to [H-3]citrulline. NOS protein expression was determined by western immunoblotting analysis. mRNA levels were examined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. EGb (50 mu g/mL) caused a 30% reduction of NO metabolites released by endothelial cells. Following EGb treatment, cellular inducible NO synthase (iNOS) activity was reduced by 28% with a concomitant reduction in the levels of iNOS protein mass and mRNA. There was no change in the activity or protein mass of constitutive NO synthase in these cells. EGb inhibited NO production by attenuating the level of iNOS mRNA in ECV304 cells. Selective inhibition of iNOS by EGb may be therapeutically relevant in modulating NO production in endothelial cells. (C) 1999 Elsevier Science Inc.
WOS关键词	LOW-DENSITY LIPOPROTEINS ; MOLECULAR-CLONING ; MOUSE MACROPHAGES ; OXIDATIVE MODIFICATION ; SELECTIVE-INHIBITION ; MESANGIAL CELLS ; ALZHEIMER-TYPE ; EGB-761 ; DEMENTIA ; BIOSYNTHESIS
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000083118100019
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274675]
专题	中国科学院上海药物研究所
通讯作者	O, K
作者单位	1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Univ Hong Kong, Fac Med, Dept Pharmacol, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Cheung, F,Siow, YL,Chen, WZ,et al. Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells[J]. BIOCHEMICAL PHARMACOLOGY,1999,58(10):1665-1673.
APA	Cheung, F,Siow, YL,Chen, WZ,&O, K.(1999).Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells.BIOCHEMICAL PHARMACOLOGY,58(10),1665-1673.
MLA	Cheung, F,et al."Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells".BIOCHEMICAL PHARMACOLOGY 58.10(1999):1665-1673.