Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures

doi:10.1046/j.1460-9568.2002.02256.x

	Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures
	Guo, H ; Tang, ZS ; Yu, Y ; Xu, L ; Jin, GZ; Zhou, JW
刊名	EUROPEAN JOURNAL OF NEUROSCIENCE
	2002-11
卷号	16 期号:10 页码:1861-1870
关键词	apomorphine dopaminergic neurotrophic factors rat tissue culture tyrosine hydroxylase
ISSN号	0953-816X
DOI	10.1046/j.1460-9568.2002.02256.x
文献子类	Article
英文摘要	Apomorphine, the catechol-derived dopamine D-1/D-2 receptor agonist, is currently in use as an antiparkinsonian drug. It has previously been reported that apomorphine was able to elicit expression of the enzyme tyrosine hydroxylase, a marker for DA neurons, in the fetal rat cerebrocortical cultures whilst in the presence of brain-derived neurotrophic factor. The present study demonstrated that treatment of fetal rat ventral mesencephalic cultures with apomorphine caused a marked increase in the number of dopaminergic neurons. The action of apomorphine can be mimicked by dopamine receptor (D-1 and D-2) agonists or blocked by preincubation with D-1/D-2 receptor antagonists. Incubation of recipient mesencephalic cultures with the conditioned medium derived from apomorphine-stimulated donor mesencephalic cultures elicited a 3.72-fold increase in the number of TH-positive neurons. Increased mRNA expression levels of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor were also found in the apomorphine-treated mesencephalic cells along with concomitant protein expression increases in the conditioned medium. Moreover, the trophic activity observed could be partially neutralized by antibodies against either brain-derived neurotrophic factor or glial cell line-derived neurotrophic factor. Cultured fetal striatal cells, but not hippocampal cells, also responded to apomorphine treatment. The membrane filtration studies revealed that both <30 kDa and >50 kDa fractions contained trophic activities. The latter characterization distinguishes them from most known neurotrophic factors. These results suggest that the apomorphine-modulated development of dopaminergic neurons may be mediated by activation of the dopamine receptor subtypes D-1 and D-2 thereby increasing the production of multiple growth factors.
WOS关键词	FIBROBLAST GROWTH-FACTOR ; TYROSINE-HYDROXYLASE EXPRESSION ; NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; SUBSTANTIA-NIGRA ; MESSENGER-RNA ; VENTRAL MESENCEPHALON ; POSTNATAL-DEVELOPMENT ; RETROGRADE TRANSPORT ; PRENATAL-DEVELOPMENT
WOS研究方向	Neurosciences & Neurology
语种	英语
出版者	BLACKWELL PUBLISHING LTD
WOS记录号	WOS:000179537800004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274314]
专题	中国科学院上海药物研究所
通讯作者	Zhou, JW
作者单位	1.Chinese Acad Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Guo, H,Tang, ZS,Yu, Y,et al. Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2002,16(10):1861-1870.
APA	Guo, H,Tang, ZS,Yu, Y,Xu, L,Jin, GZ,&Zhou, JW.(2002).Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures.EUROPEAN JOURNAL OF NEUROSCIENCE,16(10),1861-1870.
MLA	Guo, H,et al."Apomorphine induces trophic factors that support fetal rat mesencephalic dopaminergic neurons in cultures".EUROPEAN JOURNAL OF NEUROSCIENCE 16.10(2002):1861-1870.