Analysis of triptolide-regulated gene expression in Jurkat cells by complementary DNA microarray

	Analysis of triptolide-regulated gene expression in Jurkat cells by complementary DNA microarray
	Du, ZY ; Li, XY ; Li, YC ; Wang, SY
刊名	ACTA PHARMACOLOGICA SINICA
	2003-09
卷号	24 期号:9 页码:864-872
关键词	cDNA microarrays triptolide Jurkat cells gene expression
ISSN号	1671-4083
文献子类	Article
英文摘要	AIM: To investigate the global gene expression profile changes in Jurkat cells after triptolide treatment in order to find the possible triptolide targets. METHODS: Jurkat cells were treated with or without triptolide 10 mug/L for 2 h. Total RNA were isolated and used as templates for reverse transcriptional labeling of fluorescent cDNA probes. High density DNA microarray chips with a set of 13 872 human genes/Ests were used to generate the expression profile of triptolide-treated or untreated control Jurkat cells by hybridizing with fluorescent labeled probes. Array image was acquired and analyzed with array analyzing software GeneSpring. RESULTS: Triptolide significantly suppressed expression of 117 genes in Jurkat cells. Among these 117 genes, 30 % were Ests or genes without known functions, 13 % were transcription factors, 9 % were signal transduction pathway regulators, and 9 % were DNA binding proteins. Notably, the expression of mitogen-activated protein kinase kinase kinase kinase 5 (MAP kinase 5) and phosphoinositide-3-kinase (PI-3 kinase) was inhibited more than 100-fold. Moreover, the expression of genes involved in lipid transportation and metabolism was down-regulated by triptolide. CONCLUSION: High-density microarray provided an effective approach to identify drug targeting molecules. It is suggested that the widely known immune suppressive and antitumor effects of triptolide were mediated at least in part by suppression of MAP kinase and PI-3 kinase gene expression.
WOS关键词	WILFORDII HOOK F ; MALE ANTIFERTILITY COMPOUNDS ; TRIPTERYGIUM-WILFORDII ; HERBAL REMEDY ; T-CELLS
WOS研究方向	Chemistry ; Pharmacology & Pharmacy
语种	英语
CSCD记录号	CSCD:1270847
出版者	SHANGHAI INST MATERIA MEDICA
WOS记录号	WOS:000185357500005
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/274189]
专题	中国科学院上海药物研究所
通讯作者	Li, XY
作者单位	1.Chinese Acad Sci, Dept Med Chem, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dept Pharmacol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Du, ZY,Li, XY,Li, YC,et al. Analysis of triptolide-regulated gene expression in Jurkat cells by complementary DNA microarray[J]. ACTA PHARMACOLOGICA SINICA,2003,24(9):864-872.
APA	Du, ZY,Li, XY,Li, YC,&Wang, SY.(2003).Analysis of triptolide-regulated gene expression in Jurkat cells by complementary DNA microarray.ACTA PHARMACOLOGICA SINICA,24(9),864-872.
MLA	Du, ZY,et al."Analysis of triptolide-regulated gene expression in Jurkat cells by complementary DNA microarray".ACTA PHARMACOLOGICA SINICA 24.9(2003):864-872.